Genetic diversity of hepatozoon spp. In rodents from chile

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Autor(es): dc.contributorUniversidad Austral de Chile-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorRoss University School of Veterinary Medicine-
Autor(es): dc.creatorAlabí, Amir Salvador-
Autor(es): dc.creatorMonti, Gustavo-
Autor(es): dc.creatorOtth, Carola-
Autor(es): dc.creatorSepulveda-García, Paulina-
Autor(es): dc.creatorPerles, Livia-
Autor(es): dc.creatorMachado, Rosangela Zacarias-
Autor(es): dc.creatorAndré, Marcos Rogério-
Autor(es): dc.creatorBittencourt, Pedro-
Autor(es): dc.creatorMüller, Ananda-
Data de aceite: dc.date.accessioned2025-08-21T19:15:26Z-
Data de disponibilização: dc.date.available2025-08-21T19:15:26Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/S1984-29612021082-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229892-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229892-
Descrição: dc.descriptionThis study aimed to investigate the genetic diversity of Hepatozoon spp. in rodents from Valdivia, Chile. A total of 74 rodents (synanthropic n=38; wild n=36) were trapped in Valdivia. We performed conventional PCR assays for Apicomplexa organisms targeting two overlapping 18S rDNA gene fragments (600 bp and 900 bp) followed by sequencing of selected amplicons. Hepatozoon spp. occurrence was 82.43% (61/74). Twelve sequences obtained from the 600 bp and ten from the 900 bp 18S rDNA fragments were identified as Hepatozoon sp. Six sequences obtained from 18S rDNA-based overlapping PCR protocols were used for concatenated (1,400 bp) phylogenetic, haplotype and distance analyses. Hepatozoon spp. 18S rDNA concatenated sequences from the present study were detected in Oligoryzomys longicaudatus, Rattus norvegicus, Mus musculus, and Abrothrix longipilis grouped with Hepatozoon species earlier described in rodents and reptiles from Chile and Brazil. Nucleotide polymorphism of the six 18S rDNA sequences (1,400 bp) from this study, and other Chilean sequences from rodents and rodent’s ticks, showed high diversity with a total of nine Chilean haplotypes. Three haplotypes from Valdivia were identified for the first time in this study, suggesting the circulation of novel haplotypes in rodents from southern Chile.-
Descrição: dc.descriptionInstituto de Ciencias Clínicas Veterinarias Facultad de Ciencias Veterinarias Universidad Austral de Chile-
Descrição: dc.descriptionInsituto de Medicina Preventiva Veterinaria Facultad de Ciencias Veterinarias Universidad Austral de Chile-
Descrição: dc.descriptionInstituto de Microbiologia Clínica Facultad de Medicina Universidad Austral de Chile-
Descrição: dc.descriptionDepartamento de Patologia Reprodução e Saúde Única Faculdade de Ciências Agrárias e Veterinarias – FCAV Universidade Estadual Paulista – UNESP-
Descrição: dc.descriptionOne Health Center for Zoonoses and Tropical Veterinary Medicine Department of Biomedical Sciences Ross University School of Veterinary Medicine-
Descrição: dc.descriptionDepartamento de Patologia Reprodução e Saúde Única Faculdade de Ciências Agrárias e Veterinarias – FCAV Universidade Estadual Paulista – UNESP-
Idioma: dc.languageen-
Relação: dc.relationRevista Brasileira de Parasitologia Veterinaria-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectApicomplexan-
Palavras-chave: dc.subjectHepatozoonosis-
Palavras-chave: dc.subjectPCR-
Palavras-chave: dc.subjectPhylogenetics-
Palavras-chave: dc.subjectRodentia-
Palavras-chave: dc.subjectSouth america-
Título: dc.titleGenetic diversity of hepatozoon spp. In rodents from chile-
Título: dc.titleDiversidade genética de hepatozoon spp. Em roedores do chile-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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