Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorSão Carlos Federal University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorAraújo, Tamara Teodoro-
Autor(es): dc.creatorCarvalho, Thamyris Souza-
Autor(es): dc.creatorDionizio, Aline-
Autor(es): dc.creatorDebortolli, Ana Luiza Bogaz-
Autor(es): dc.creatorVentura, Talita Mendes Oliveira-
Autor(es): dc.creatorSouza, Beatriz Martines-
Autor(es): dc.creatorFeitosa, Chelsea Maria Vilas Boas-
Autor(es): dc.creatorBarbosa, Heloisa Aparecida Pereira-
Autor(es): dc.creatorRibeiro, Caroline Fernanda da Silva-
Autor(es): dc.creatorMartini, Tatiana-
Autor(es): dc.creatorTaira, Even-
Autor(es): dc.creatorShibao, Priscila Yumi Tanaka-
Autor(es): dc.creatorHenrique-Silva, Flavio-
Autor(es): dc.creatorMarchetto, Reinaldo-
Autor(es): dc.creatorBuzalaf, Marília Afonso Rabelo-
Data de aceite: dc.date.accessioned2025-08-21T16:29:48Z-
Data de disponibilização: dc.date.available2025-08-21T16:29:48Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2021.103874-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229891-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229891-
Descrição: dc.descriptionObjective: To study the proteomic alterations in the initial AEP after rinsing with CaneCPI-5, StN15 or Hb or their combination. Materials and methods: In five crossover phases, after prophylaxis, 10 volunteers in 5 consecutive days, rinsed (10 mL, 1 min) with the following solutions: deionized water (H2O- negative control- 1), 0.1 mg/mL CaneCPI-5 (2), 1.88×10−5 M StN15 (3), 1.0 mg/mL Hb (4) or their combination (5). The AEP formed after 3 min was collected with electrode filter papers soaked in 3% citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. Results: Rinsing with the proteins/peptide increased the amounts of proteins in the AEP. The total numbers of proteins identified after rinsing with CaneCPI-5, StN15, Hb or their combination versus water, were 131, 167, 148 and 142, respectively. The treatment with the proteins/peptide or their combination increased proteins that bind calcium, phosphate and interact with distinct proteins, as well as proteins with antimicrobial and acid-resistant properties, such as, Cornifin-B (7.7, 12.6, and 4.3-fold for CaneCPI-5, StN15 and Hb, respectively), isoforms of Cystatin (2.2–2.4-fold for CaneCPI-5 and StN15), Proline-rich-protein 4 (4.3-fold; StN15), Histatin-1 (2.8-fold; StN15) and Hemoglobin (7.7–25-fold for Hb and Combination). Immunoglobulin, Keratin and Histone were exclusively identified upon treatment with the proteins/peptide, alone or combined. Conclusion: Rinsing with proteins/peptide, alone or combined, increased protective proteins in the initial AEP. Clinical Relevance: Our results suggest that rinsing with the proteins/peptide or their combination increases the proteins capable of enhancing the protective function of the basal layer of AEP.-
Descrição: dc.descriptionDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo-
Descrição: dc.descriptionDepartment of Genetics and Evolution São Carlos Federal University-
Descrição: dc.descriptionDepartment of Biochemistry and Technology Institute of Chemistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biochemistry and Technology Institute of Chemistry São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationJournal of Dentistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAcquired enamel pellicle-
Palavras-chave: dc.subjectCaneCPI-5-
Palavras-chave: dc.subjectHemoglobin-
Palavras-chave: dc.subjectProteomics-
Palavras-chave: dc.subjectStatherin-
Título: dc.titleProtein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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