Intradermal comparative cervical tuberculin test in the diagnosis of caprine tuberculosis

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorSecretariat of Agriculture and Supply of State of São Paulo-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorAdolfo Lutz Institute-
Autor(es): dc.contributorUFCG-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorde Almeida, Carlos Augusto Scacchetti-
Autor(es): dc.creatordos Santos, Caio R.-
Autor(es): dc.creatorBenites, Nilson Roberti-
Autor(es): dc.creatorJordão, Ricardo S.-
Autor(es): dc.creatorChimara, Érica-
Autor(es): dc.creatorBenesi, Fernando José-
Autor(es): dc.creatorde Azevedo, Sergio S.-
Autor(es): dc.creatorRibeiro, Márcio Garcia-
Autor(es): dc.creatorPinheiro, Sônia Regina-
Data de aceite: dc.date.accessioned2025-08-21T15:55:13Z-
Data de disponibilização: dc.date.available2025-08-21T15:55:13Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s42770-021-00650-9-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229877-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229877-
Descrição: dc.descriptionTuberculosis remains one of the most important infectious diseases with well-known zoonotic nature that affect humans, wildlife, and domestic animals, including goats. Nonetheless, no intradermal tuberculin test has been standardized for caprine diagnosis of tuberculosis. The present study investigated the intradermal comparative cervical tuberculin test (ICCTT) in the diagnosis of tuberculosis among 60 goats from farms with history of tuberculosis. The cutoff applied to goats was based on a study where goats had been experimentally infected with Mycobacterium bovis and Mycobacterium avium. Clinical examination, bacteriological culture, and histopathological staining were assessed to the diagnosis. Isolates compatible with mycobacteria were subjected for molecular diagnosis based on gyrB-restriction fragment length polymorphism (RFLP) analysis and PCR restriction-enzyme analysis (PRA) of hsp65 gene by BstEII and HaeIII, namely PRA-hsp65 assay. From all goats, 60% (n = 36/60), 3.3% (n = 2/60), and 36.7% (n = 22/60) showed positive, inconclusive, and negative reactions, respectively. Out of 36 goats with ICCTT positive, 75% (n = 27/36) had isolation of mycobacteria and were detected M. bovis by gyrB-RFLP. Molecular diagnosis and histopathological findings compatible with tuberculosis showed 86.1% (n = 31/36) concordance with the ICCTT. When compared ICCTT with M. bovis isolation, gyrB-RFLP, and histopathology, the better arithmetic means of sensitivity and specificity were 2.5 mm for ICCTT compared with M. bovis isolation and gyrB-RFLP, and 4.55 mm when compared with histopathology. Both receiver operating characteristic (ROC) curves presented statistical significance (P < 0.001). The identification of other mycobacteria, e.g., M. kansasii, M. flavescens, M. avium, M. florentinum, M. lentiflavum, M. simiae, and Corynebacterium pseudotuberculosis, not influenced positive results in ICCTT. The concordance between bacteriological, histopathological, and molecular identification with ICCTT findings indicate that the tuberculin test may be used as a valuable tool for diagnosis of caprine tuberculosis and reinforce the importance of association of methods to diagnostic of the disease from animal origin.-
Descrição: dc.descriptionCoordination of Sustainable Rural Development Secretariat of Agriculture and Supply of State of São Paulo, SP-
Descrição: dc.descriptionAnimal Pathology Department School of Veterinary Medicine and Animal Sciences University of São Paulo-USP, SP-
Descrição: dc.descriptionDepartment of Preventive Veterinary Medicine and Animal Science USP, SP-
Descrição: dc.descriptionBiological Institute of São Paulo Secretariat of Agriculture and Supply of State of São Paulo, SP-
Descrição: dc.descriptionTuberculosis and Mycobacteriosies Laboratory Adolfo Lutz Institute, SP-
Descrição: dc.descriptionDepartament of Clinics USP, SP-
Descrição: dc.descriptionVeterinary Medicine Academic Unit Federal University of Campina Grande UFCG, PB-
Descrição: dc.descriptionDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences São Paulo State University-UNESP, SP-
Descrição: dc.descriptionDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences São Paulo State University-UNESP, SP-
Formato: dc.format421-431-
Idioma: dc.languageen-
Relação: dc.relationBrazilian Journal of Microbiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectGoats-
Palavras-chave: dc.subjectgyrB-RFLP-
Palavras-chave: dc.subjectMycobacterium bovis-
Palavras-chave: dc.subjectPRA-hsp65-
Palavras-chave: dc.subjectTuberculin tests-
Título: dc.titleIntradermal comparative cervical tuberculin test in the diagnosis of caprine tuberculosis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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