An affordable and efficienthomemade platform for drosophila behavioral studies, and an accompanying protocol for larval mitochondrial respirometry

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade do Oeste Paulista-
Autor(es): dc.creatorGarcia, Geovana S.-
Autor(es): dc.creatorOthonicar, Murilo F.-
Autor(es): dc.creatorOliveira, Marcos T.-
Autor(es): dc.creatorCouto-Lima, Carlos A.-
Data de aceite: dc.date.accessioned2025-08-21T20:08:56Z-
Data de disponibilização: dc.date.available2025-08-21T20:08:56Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3791/62669-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229836-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229836-
Descrição: dc.descriptionThe usefulness of Drosophila as a model organism for the study of human diseases, behaviors and basic biology is unquestionable. Although practical, Drosophila research lacks popularity in developing countries, possibly due to the misinformed idea that establishing a lab and performing relevant experiments with such tiny insects is difficult and requires expensive, specialized apparatuses. Here, we describe how to build an affordable flylab to quantitatively analyze a myriad of behavioral parameters in D. melanogaster, by 3D-printing many of the necessary pieces of equipment. We provide protocols to build in-house vial racks, courtship arenas, apparatuses for locomotor assays, etc., to be used for general fly maintenance and to perform behavioral experiments using adult flies and larvae. We also provide protocols on how to use more sophisticated systems, such as a high resolution oxygraph, to measure mitochondrial oxygen consumption in larval samples, and show its association with behavioral changes in the larvae upon the xenotopic expression of the mitochondrial alternative oxidase (AOX). AOX increases larval activity and mitochondrial leak respiration, and accelerates development at low temperatures, which is consistent with a thermogenic role for the enzyme. We hope these protocols will inspire researchers, especially from developing countries, to use Drosophila to easily combine behavior and mitochondrial metabolism data, which may lead to information on genes and/or environmental conditions that may also regulate human physiology and disease states.-
Descrição: dc.descriptionDepartamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista “Júlio de Mesquita Filho”-
Descrição: dc.descriptionFaculdade de Ciências da Saúde Biomedicina Universidade do Oeste Paulista-
Descrição: dc.descriptionDepartamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista “Júlio de Mesquita Filho”-
Idioma: dc.languageen-
Relação: dc.relationJournal of Visualized Experiments-
???dc.source???: dc.sourceScopus-
Título: dc.titleAn affordable and efficienthomemade platform for drosophila behavioral studies, and an accompanying protocol for larval mitochondrial respirometry-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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