In vitro evaluation of acellular collagen matrices derived from porcine pericardium: Influence of the sterilization method on its biological properties

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorDe Grandis, Rone Aparecido-
Autor(es): dc.creatorMiotto, Larissa Natiele-
Autor(es): dc.creatorGenaro, Luis Eduardo-
Autor(es): dc.creatorPolli, Larissa Migliatti-
Autor(es): dc.creatorPlepis, Ana Maria de Guzzi-
Autor(es): dc.creatorRodrigues, Fabiana Tessari-
Autor(es): dc.creatorMartins, Virginia da Conceição Amaro-
Autor(es): dc.creatorFranchi, Leonardo Pereira-
Autor(es): dc.creatorScarel-Caminaga, Raquel Mantuaneli-
Autor(es): dc.creatorCapote, Ticiana Sidorenko de Oliveira-
Data de aceite: dc.date.accessioned2025-08-21T23:29:48Z-
Data de disponibilização: dc.date.available2025-08-21T23:29:48Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ma14216255-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229777-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229777-
Descrição: dc.descriptionThe aim of this study were characterize acellular collagen matrices derived from porcine pericardium (PP) and to evaluate their properties after sterilization by ethylene oxide and gamma ray. PP matrices were subjected to alkaline hydrolysis (AH), and samples were characterized for biological stability, membrane thickness measurements, differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). Subsequently, the matrices were frozen, lyophilized and sterilized by ethylene oxide or gamma radiation. For in vitro assays, CHO-K1 cell culture was used and evaluated for cytotoxicity, clonogenic survival assay, genotoxicity and mutagenicity. Analysis of variance (ANOVA) was used, followed by Dunnett’s post-test, with a significance level of 5%. After AH, there was no significant change in matrix thickness. The relative biodegradability of the material after implantation was observed. Morphology and dimensions had small changes after AH. As for cell viability, none of the tested matrices showed a statistically significant difference (p > 0.05; Dunnett) regardless of the sterilization method. Furthermore, it was found that PP matrices did not interfere with the proliferation capacity of CHO-K1 cells (p > 0.05; Dunnett). As for genotoxicity, when sterilized with ethylene oxide (NP, P12 and P24), it showed genotoxic potential, but it was not genotoxic when sterilized by gamma radiation. No mutagenic effects were observed in either group. PP-derived collagen matrices hydrolyzed at different times were not cytotoxic. It is concluded that the best method of sterilization is through gamma radiation, since no significant changes were observed in the properties of the PP matrices.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionFaculty of Pharmaceutical Sciences-FCFar Paulista State University-
Descrição: dc.descriptionDepartment of Morphology Genetics Orthodontic and Pediatric Dentistry Araraquara School of Dentistry Paulista State University-
Descrição: dc.descriptionDepartment of Chemistry and Molecular Physics Institute of Chemistry of São Carlos University of São Paulo-
Descrição: dc.descriptionDepartment of Genetics Faculty of Medicine of Ribeirão Preto University of São Paulo-
Descrição: dc.descriptionFaculty of Pharmaceutical Sciences-FCFar Paulista State University-
Descrição: dc.descriptionDepartment of Morphology Genetics Orthodontic and Pediatric Dentistry Araraquara School of Dentistry Paulista State University-
Descrição: dc.descriptionFAPESP: 2011/17723-0-
Descrição: dc.descriptionFAPESP: 2021/10095-5-
Idioma: dc.languageen-
Relação: dc.relationMaterials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCollagen matrices-
Palavras-chave: dc.subjectPorcine pericardium-
Palavras-chave: dc.subjectTissue regeneration-
Título: dc.titleIn vitro evaluation of acellular collagen matrices derived from porcine pericardium: Influence of the sterilization method on its biological properties-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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