Specific parameters of infrared LED irradiation promote the inhibition of oxidative stress in dental pulp cells

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal de Uberlândia (UFU)-
Autor(es): dc.contributorUNAERP-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorBonvicini, Jéssica F.S.-
Autor(es): dc.creatorde Souza, Gabriela L.-
Autor(es): dc.creatorBasso, Fernanda G.-
Autor(es): dc.creatorde Souza Costa, Carlos A.-
Autor(es): dc.creatorMoura, Camilla C.G.-
Autor(es): dc.creatorSilva, Marcelo J.B.-
Autor(es): dc.creatorTurrioni, Ana Paula-
Data de aceite: dc.date.accessioned2025-08-21T15:54:58Z-
Data de disponibilização: dc.date.available2025-08-21T15:54:58Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2021.105273-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229702-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229702-
Descrição: dc.descriptionObjectives: The present study aimed to assess the oxidative stress and the viability of dental pulp cells stimulated by lipopolysaccharide (LPS) and submitted to photobiomodulation (PBM) with infrared light-emitting diode (LED, 850 nm). Design: Three healthy primary teeth (n = 3) were collected and seeded in 24-well plates with 10 µg/mL of LPS to induce inflammatory mediator formation. The cells were irradiated (850 nm, 40 mW/cm2 and 80 mW/cm2) at the proposed radiant exposures of 0 (control), 4, 15, and 30 J/cm2 shortly after LPS supplementation. The tests were performed 24 h after irradiation to assess mitochondrial activity (MTT assay), the number of viable cells (Trypan Blue), cell morphology (Scanning Electron Microscopy - SEM), and the quantification of Nitric Oxide (NO) and Reactive Oxygen Species (ROS). The data were analyzed using Kruskal–Wallis and Dunn's tests (p < 0.05). Results: The irradiated groups showed larger viable cells number than the non-irradiated group with LPS (p < 0.0001). All irradiation parameters decreased ROS concentrations after LPS application compared to the non-irradiated group (p < 0.05). All irradiation parameters enhanced the NO values compared to those of the control group (p < 0.05). The SEM images showed cells with regular morphology that adhered to the substrate. Conclusions: According to the parameters used in this study, the radiant exposure of 15 J/cm2 and irradiance of 40 mW/cm2 were the most effective irradiation parameters to stimulate and modulate oxidative stress in the primary teeth-derived dental pulp cells.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Pediatric Dentistry School of Dentistry Federal University of Uberlândia UFU-
Descrição: dc.descriptionDepartment of Endodontics School of Dentistry Federal University of Uberlandia UFU-
Descrição: dc.descriptionDepartment of Oral Pathology University of Ribeirão Preto UNAERP-
Descrição: dc.descriptionDepartment of Physiology and Pathology Araraquara School of Dentistry UNESP-
Descrição: dc.descriptionInstitute of Biomedical Sciences Federal University of Uberlandia UFU-
Descrição: dc.descriptionDepartment of Physiology and Pathology Araraquara School of Dentistry UNESP-
Descrição: dc.descriptionCNPq: 434204-2018-8-
Idioma: dc.languageen-
Relação: dc.relationArchives of Oral Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDental pulp cells-
Palavras-chave: dc.subjectInfrared LED-
Palavras-chave: dc.subjectIrradiation-
Palavras-chave: dc.subjectReactive oxygen species-
Título: dc.titleSpecific parameters of infrared LED irradiation promote the inhibition of oxidative stress in dental pulp cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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