Miniaturized Microbiological Method to Determine the Potency of Rifaximin in Tablets

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.creatorCorrea, Clara Bersi Motta-
Autor(es): dc.creatorKogawa, Ana Carolina-
Autor(es): dc.creatorChorilli, Marlus-
Autor(es): dc.creatorSalgado, Hérida Regina Nunes-
Data de aceite: dc.date.accessioned2025-08-21T16:03:11Z-
Data de disponibilização: dc.date.available2025-08-21T16:03:11Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-08-20-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/jaoacint/qsab017-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229694-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229694-
Descrição: dc.descriptionBACKGROUND: Rifaximin, a semi-synthetic antimicrobial, does not present microbiological method described in official compendia, and there is a lack of literature on this topic. The quality control of antimicrobials is extremely important to evaluate the real potency of pharmaceutical products. OBJECTIVE: A miniaturized turbidimetric method for determining the potency of rifaximin in tablets was developed and validated by turbidimetry, according to the international guidelines. METHOD: Escherichia coli ATCC 10536 IAL 2393, brain heart infusion (BHI) broth, inoculum at 8%, rifaximin in purified water with 20% ethanol at 5, 10, and 20 μg/mL and 530 nm were used. RESULTS: The method was considered selective for rifaximin, as the adjuvants did not show activity; linear with correlation coefficients 0.9998 for standard and 0.9999 for sample; accurate with 99.73% recovery; precise with RSD less than 3%; and robust in the face of small variations in (i) rifaximin volume, (ii) proportion of ethanol, (iii) inoculum volume. CONCLUSIONS: The method is considered adequate and safe to evaluate the potency of rifaximin in tablets, contemplating speed, low cost, low waste generation, and ease of operation. HIGHLIGHTS: This work usescurrent, sustainable, and green analytical chemistry and can be used in the routine analyses of rifaximin by laboratories and the pharmaceutical industry around the world.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University School of Pharmaceutical Sciences Department of Drugs and Medicines-
Descrição: dc.descriptionUniversidade Federal de Goiás Faculdade de Farmácia Laboratório de Controle de Qualidade-
Descrição: dc.descriptionSão Paulo State University School of Pharmaceutical Sciences Department of Drugs and Medicines-
Formato: dc.format1049-1054-
Idioma: dc.languageen-
Relação: dc.relationJournal of AOAC International-
???dc.source???: dc.sourceScopus-
Título: dc.titleMiniaturized Microbiological Method to Determine the Potency of Rifaximin in Tablets-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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