IL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot study

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Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorStolf, Camila S.-
Autor(es): dc.creatorSacramento, Catharina M.-
Autor(es): dc.creatorPaz, Hélvis E. S.-
Autor(es): dc.creatorMachado, Renato A.-
Autor(es): dc.creatorRamos, Lucas P.-
Autor(es): dc.creatorde Oliveira, Luciane D.-
Autor(es): dc.creatorCogo-Müller, Karina-
Autor(es): dc.creatorSantamaria, Mauro P.-
Autor(es): dc.creatorRuiz, Karina G. S.-
Autor(es): dc.creatorCasarin, Renato C. V.-
Data de aceite: dc.date.accessioned2025-08-21T15:45:38Z-
Data de disponibilização: dc.date.available2025-08-21T15:45:38Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/jre.12940-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229641-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229641-
Descrição: dc.descriptionBackground and objective: Previous studies have demonstrated an association between the IL10 promoter rs6667202 (C > A) single-nucleotide polymorphism (SNP) and grade C, stage 3 or 4 periodontitis (Perio4C) in the Brazilian population, where the altered A allele was detected more frequently in these patients. However, no functional analysis of this variation has yet been performed. Thus, the objective of this preliminary study was to evaluate the functionality of rs6667202 in gingival fibroblasts (GFs) of individuals with Perio4C and with periodontal health (PH) stimulated with Aggregatibacter actinomycetencomitans protein extract (AaPE). Methods: Patients with PH and Perio4C were segregated according to their genotype (AA, AC, or CC), and a biopsy was performed to establish the culture of the GFs. After GFs exposure to AaPE at 5 µg/ml for 1.5 h, RNA was extracted to analyze IL10 expression by qPCR. Aliquots of the cell's supernatant were subjected to immunoenzymatic analysis (MAGpix) to detect interleukin-10 (IL-10). Results: In PH, the genotypes AA and AC are related to less expression of IL10 (p = 0.027 and p < 0.0001) and less production of IL-10 (p = 0.002 and p = 0.001), when compared to CC. In Perio4C, there was no statistical difference between the genotypes (p > 0.05), although a lower IL-10 expression and release compared with PH CC was seen (p = 0.033 and p < 0.001). Conclusion: The rs6667202 SNP is functional in PH, as it decreases the expression and production of IL-10. In Perio4C, other factors may be masking its action by altering the IL-10's response.-
Descrição: dc.descriptionPeriodontics Division Department of Prosthodontics and Periodontics Piracicaba Dental School University of Campinas-
Descrição: dc.descriptionOral Pathology Division Department of Oral Diagnosis Piracicaba Dental School University of Campinas-
Descrição: dc.descriptionMicrobiology and Immunology Division Department of Biosciences and Oral Biopathology São José dos Campos School of Dentistry São Paulo State University-
Descrição: dc.descriptionPharmacology Anesthesiology and Therapeutics Division Department of Biosciences Piracicaba Dental School University of Campinas-
Descrição: dc.descriptionPeriodontics Division Department of Diagnosis and Surgery São José dos Campos School of Dentistry São Paulo State University-
Descrição: dc.descriptionMicrobiology and Immunology Division Department of Biosciences and Oral Biopathology São José dos Campos School of Dentistry São Paulo State University-
Descrição: dc.descriptionPeriodontics Division Department of Diagnosis and Surgery São José dos Campos School of Dentistry São Paulo State University-
Formato: dc.format85-93-
Idioma: dc.languageen-
Relação: dc.relationJournal of Periodontal Research-
???dc.source???: dc.sourceScopus-
Título: dc.titleIL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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