Interaction of epigallocatechin-gallate and chlorhexidine with Streptococcus mutans stimulated odontoblast-like cells: Cytotoxicity, Interleukin-1β and co-species proteomic analyses

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Autor(es): dc.contributorUniversity of Toronto-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorStavroullakis, Alexander Terry-
Autor(es): dc.creatorGoncalves, Lucelia Lemes-
Autor(es): dc.creatorLevesque, Celine Marie-
Autor(es): dc.creatorKishen, Anil-
Autor(es): dc.creatorPrakki, Anuradha-
Data de aceite: dc.date.accessioned2025-08-21T22:18:43Z-
Data de disponibilização: dc.date.available2025-08-21T22:18:43Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2021.105268-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229576-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229576-
Descrição: dc.descriptionObjectives: The dentin therapeutic agent chlorhexidine has inflammatory and cytotoxic characteristics urging investigation of alternatives like the natural compound epigallocatechin-gallate. The aim is to verify the effect of epigallocatechin-gallate and chlorhexidine on viability, interleukin-1β (IL-1β) and differential protein expression of MDPC-23 odontoblast-like cells stimulated by Streptococcus mutans. Design: Cells were stimulated with heat-killed S. mutans at multiplicity of infection (MOI) of 100–1000 and subsequently treated with 100–1 µM of epigallocatechin-gallate. Cells with no treatment or chlorhexidine were controls. Combined stimulated/treated cells were tested for cytotoxicity (Alamar-Blue, N = 3, n = 3), total protein (N = 3, n = 3), IL-1β (ELISA, N = 3, n = 3), and differential protein expression by liquid chromatography-tandem mass spectrometry (LC-MS/MS, n = 2). Results: Cells stimulated at MOI 100/1000 and treated with 10 µM epigallocatechin-gallate and chlorhexidine did not present cytotoxicity. IL-1β significantly increased in both un-stimulated and stimulated chlorhexidine 10 µM groups when compared to un-treated control (p < 0.05). MOI 100 chlorhexidine 10 µM group significantly increased IL-1β compared to un-stimulated chlorhexidine 10 µM and epigallocatechin-gallate 10 µM groups, as well as to MOI 100 epigallocatechin-gallate 10 µM group (p < 0.05). LC-MS/MS revealed S. mutans and mammalian proteins, with tooth-specific proteins exhibiting different abundance levels, depending on the tested condition. Conclusions: Odontoblast-like cells stimulated with S. mutans at different MOI combined with epigallocatechin-gallate treatment did not cause cytotoxicity. S. mutans stimulation combined with chlorhexidine 100 µM treatment decreased cell viability, while treatment with chlorhexidine 10 µM concentration significantly increased IL-1β. S. mutans stimulation and treatment of cells resulted in varied protein expression.-
Descrição: dc.descriptionDesert Research Institute-
Descrição: dc.descriptionNatural Sciences and Engineering Research Council of Canada-
Descrição: dc.descriptionDepartment of Clinical Sciences – Restorative Faculty of Dentistry University of Toronto-
Descrição: dc.descriptionDepartment of Restorative Dentistry Institute of Science and Technology of São José dos Campos Sao Paulo State University-
Descrição: dc.descriptionDepartment of Biological and Diagnostic Sciences-Oral Microbiology Faculty of Dentistry University of Toronto-
Descrição: dc.descriptionDental Research Institute Faculty of Dentistry University of Toronto-
Descrição: dc.descriptionDepartment of Restorative Dentistry Institute of Science and Technology of São José dos Campos Sao Paulo State University-
Descrição: dc.descriptionNatural Sciences and Engineering Research Council of Canada: 2018-06489-
Idioma: dc.languageen-
Relação: dc.relationArchives of Oral Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectChlorhexidine-
Palavras-chave: dc.subjectEGCG-
Palavras-chave: dc.subjectIL-1β-
Palavras-chave: dc.subjectLC-MS/MS-
Palavras-chave: dc.subjectMDPC-23-
Palavras-chave: dc.subjectS. mutans-
Título: dc.titleInteraction of epigallocatechin-gallate and chlorhexidine with Streptococcus mutans stimulated odontoblast-like cells: Cytotoxicity, Interleukin-1β and co-species proteomic analyses-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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