Liquid biopsy can detect BRCA2 gene variants in female dogs with mammary neoplasia

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFAMERP-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorde Oliveira, Jéssica Rodrigues-
Autor(es): dc.creatorColombo, Jucimara-
Autor(es): dc.creatorGonçalves, Francine Moraes-
Autor(es): dc.creatorde Carvalho, Lucas Amoroso Lopes-
Autor(es): dc.creatorCosta, Douglas Santos-
Autor(es): dc.creatorHenrique, Tiago-
Autor(es): dc.creatorNovais, Adriana Alonso-
Autor(es): dc.creatorMoschetta-Pinheiro, Marina Gobbe-
Autor(es): dc.creatorChuffa, Luiz Gustavo de Almeida-
Autor(es): dc.creatorCoutinho, Luiz Lehmann-
Autor(es): dc.creatorSantana, Áureo Evangelista-
Autor(es): dc.creatorZuccari, Debora Aparecida Pires de Campos-
Data de aceite: dc.date.accessioned2025-08-21T18:48:07Z-
Data de disponibilização: dc.date.available2025-08-21T18:48:07Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/vco.12758-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229550-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229550-
Descrição: dc.descriptionMammary tumours (MT) are one of the most prevalent malignancies in female dogs and women. Currently, molecular analyzes have shown that each tumour type presents its own genetic signature. In this context, liquid biopsy allows a comprehensive genetic characterisation of the tumour, enabling early diagnosis and personalised treatment of patients. In women, deleterious mutations inherited in BRCA2 gene are associated with an increased risk of breast cancer, resistance to therapies and worse prognosis. In female dogs, there are many divergent data on the involvement of BRCA2 gene with mammary carcinogenesis and what its pathogenic potential is. Therefore, the objective was to identify BRCA2 gene variants in 20 plasma DNA samples, from 10 newly diagnosed dogs with mammary cancer (RD), five control (CTR) and five mastectomized patients. Eleven single nucleotide polymorphisms (SNPs) were detected, most of them in the exon 11 and two indels (deletion/insertion) in the BRCA2 gene. However, there was no statistically significant difference in the SNPs/indels detected between the groups. In addition, only one SNP (p.T1425P) and one deletion (p.L2307del) were considered deleterious using in silico computational models. Interestingly, most common variants were present in the plasma of all groups, except for the Ile2614Thr, Ile2614Val, Thr1425Pro and p.L2307del variants. Thus, we observed that SNPs are common in the BRCA2 gene of female dogs with MT, with a similar condition identified in women with breast cancer. Liquid biopsy approach in dogs with MT is useful for genetic and therapeutic proposals.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Veterinary Clinic and Surgery School of Agricultural and Veterinarian Sciences Univ. Estadual Paulista Jaboticabal-
Descrição: dc.descriptionDepartment of Molecular Biology Cancer Molecular Research Laboratory (LIMC) FAMERP-
Descrição: dc.descriptionDepartment of Molecular Biology Molecular Markers and Bioinformatics Laboratory FAMERP-
Descrição: dc.descriptionDepartment of Anatomy-IBB/UNESP Institute of Biosciences of Botucatu Univ. Estadual Paulista Botucatu-
Descrição: dc.descriptionLuiz de Queiroz College of Agriculture (ESALQ) University of São Paulo Piracicaba-
Descrição: dc.descriptionDepartment of Molecular Biology FAMERP-
Descrição: dc.descriptionCollaborator in Program of Post-Graduate in Genetics UNESP/IBILCE-
Descrição: dc.descriptionDepartment of Veterinary Clinic and Surgery School of Agricultural and Veterinarian Sciences Univ. Estadual Paulista Jaboticabal-
Descrição: dc.descriptionDepartment of Anatomy-IBB/UNESP Institute of Biosciences of Botucatu Univ. Estadual Paulista Botucatu-
Descrição: dc.descriptionCollaborator in Program of Post-Graduate in Genetics UNESP/IBILCE-
Descrição: dc.descriptionFAPESP: 2017/15006-5-
Formato: dc.format164-171-
Idioma: dc.languageen-
Relação: dc.relationVeterinary and Comparative Oncology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBRCA2 gene-
Palavras-chave: dc.subjectbreast cancer-
Palavras-chave: dc.subjectdogs-
Palavras-chave: dc.subjectgenetic variants-
Palavras-chave: dc.subjectpersonalised medicine-
Título: dc.titleLiquid biopsy can detect BRCA2 gene variants in female dogs with mammary neoplasia-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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