Preventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Western São Paulo (UNOESTE)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorNorthern University of Paraná-
Autor(es): dc.creatorMariano, Thaoan Bruno-
Autor(es): dc.creatorDe Souza Castilho, Anthony César-
Autor(es): dc.creatorDe Almeida Sabela, Ana Karenina Dias-
Autor(es): dc.creatorDe Oliveira, André Casanova-
Autor(es): dc.creatorCury, Sarah Santiloni-
Autor(es): dc.creatorAguiar, Andreo Fernando-
Autor(es): dc.creatorDe Jesus Dutra Dias, Raisa-
Autor(es): dc.creatorCicogna, Antonio Carlos-
Autor(es): dc.creatorOkoshi, Katashi-
Autor(es): dc.creatorJustulin, Luis Antonio-
Autor(es): dc.creatorCarvalho, Robson Francisco-
Autor(es): dc.creatorPacagnelli, Francis Lopes-
Data de aceite: dc.date.accessioned2025-08-21T15:40:06Z-
Data de disponibilização: dc.date.available2025-08-21T15:40:06Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0244768-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229497-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229497-
Descrição: dc.descriptionTo gain insight on the impact of preventive exercise during pulmonary arterial hypertension (PAH), we evaluated the gene expression of myosins and gene-encoding proteins associated with the extracellular matrix remodeling of right hypertrophied ventricles. We used 32 male Wistar rats, separated in four groups: Sedentary Control (S, n = 8); Control with Training (T, n = 8); Sedentary with Pulmonary Arterial Hypertension (SPAH, n = 8); and Pulmonary Arterial Hypertension with Training (TPAH, n = 8). All rats underwent a two-week adaptation period; T and TPAH group rats then proceeded to an eight-week training period on a treadmill. At the beginning of the 11th week, S and T groups received an intraperitoneal injection of saline, and SPAH and TPAH groups received an injection of monocrotaline (60 mg/kg). Rats in the T and TPAH groups then continued with the training protocol until the 13th week. We assessed exercise capacity, echocardiography analysis, Fulton’s index, cross-sectional areas of cardiomyocytes, collagen content and types, and fractal dimension (FD). Transcript abundance of myosins and extracellular matrix genes were estimated through reverse transcription-quantitative PCR (RT-qPCR). When compared to the SPAH group, the TPAH group showed increases in functional capacity and pulmonary artery acceleration time/pulmonary ejection time ratio and decreases in Fulton’s index and cross-sectional areas of myocyte cells. However, preventive exercise did not induce alterations in col1a1 and myh7 gene expression. Our findings demonstrate that preventive exercise improved functional capacity, reduced cardiac hypertrophy, and attenuated PH development without interfering in mRNA-encoding myosin and collagen expression during PAH.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionPostgraduate Program in Animal Science University of Western São Paulo (UNOESTE), Presidente Prudente-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences UNESP, Botucatu-
Descrição: dc.descriptionPostgraduate Program in Physical Exercise in Health Promotion Northern University of Paraná, Paraná-
Descrição: dc.descriptionDepartment of Physiotherapy University of Western São Paulo (UNOESTE), Presidente Prudente-
Descrição: dc.descriptionDepartment of Internal Medicine Botucatu Medical School UNESP, Botucatu-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences UNESP, Botucatu-
Descrição: dc.descriptionDepartment of Internal Medicine Botucatu Medical School UNESP, Botucatu-
Descrição: dc.descriptionFAPESP: #2016/11344-0-
Descrição: dc.descriptionFAPESP: #2018/12526-0-
Descrição: dc.descriptionFAPESP: #2018/24317-7-
Idioma: dc.languageen-
Relação: dc.relationPLoS ONE-
???dc.source???: dc.sourceScopus-
Título: dc.titlePreventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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