Rational design of nanocarriers based on gellan gum/retrograded starch exploiting polyelectrolyte complexation and ionic cross-linking processes: A potential technological platform for oral delivery of bevacizumab

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorCardoso, Valéria Maria de Oliveira-
Autor(es): dc.creatorKiraly, Vanessa Thomaz Rodrigues-
Autor(es): dc.creatorBoni, Fernanda Isadora-
Autor(es): dc.creatorFerreira, Natália Noronha-
Autor(es): dc.creatorFerreira, Leonardo M.B.-
Autor(es): dc.creatorPereira, Fabíola Manhas Verbi-
Autor(es): dc.creatorBorges, Júlio César-
Autor(es): dc.creatorCury, Beatriz Stringhetti Ferreira-
Autor(es): dc.creatorGremião, Maria Palmira Daflon-
Data de aceite: dc.date.accessioned2025-08-21T17:11:51Z-
Data de disponibilização: dc.date.available2025-08-21T17:11:51Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jddst.2021.102765-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229451-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229451-
Descrição: dc.descriptionThe structural fragility of monoclonal antibodies (mAbs), such as bevacizumab (BVZ), is a critical parameter for oral administration and can limit the use of several technologies to produce oral nanocarriers for these biomolecules. Nanoparticles (NPs) based on gellan gum (GG) and retrograded starch (RS) were rationally designed through polyelectrolyte complexation, and ionic cross-linking was exploited as an additional technological strategy to modulate the properties of nanocarriers. According to static light scattering analysis, the molecular weights of GG and RS were approximately 158 kDa and 1803 kDa, respectively. The influence of pH on the zeta potential (ZP) of polymers allowed the selection of pH 6.2 as the most suitable pH for the complexation of these polyelectrolytes. Non-cross-linked NPs were prepared at different polymer:drug ratios and the effects of formulation variables (polyelectrolyte ratio, drug and cross-linker concentrations, and polymer:drug ratio) on the formation and properties (size, ZP, and PDI) of cross-linked NPs were evaluated using a 33 full-factorial design. The average size of non-cross-linked and cross-linked NPs ranged from 260.1 - 299.6 nm to 265.7–629.9 nm, respectively. NPs-negative ZP (>- 20 mV) and high association efficiency (AE%) (>56.16%) were achieved. Cross-linking significantly increased the BVZ AE% (85%–100%). Analyses by attenuated total reflectance-Fourier transform infrared, fluorescence, circular dichroism, and differential scanning microcalorimetry techniques demonstrated that polyelectrolyte complexation and ionic cross-linking did not denature the secondary and tertiary structures of BVZ. The results revealed the suitability of the technological approaches used to produce BVZ-loaded nanocarriers with tailored properties, representing a potential platform for the oral delivery of BVZ.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUNESP - São Paulo State University School of Pharmaceutical Sciences Department of Drugs and Medicines-
Descrição: dc.descriptionUNESP - São Paulo State University Institute of Chemistry Department of Analytical Chemistry Physical Chemistry and Inorganic-
Descrição: dc.descriptionUSP - São Paulo University São Carlos Institute of Chemistry Department of Chemistry and Molecular Physics-
Descrição: dc.descriptionUNESP - São Paulo State University School of Pharmaceutical Sciences Department of Drugs and Medicines-
Descrição: dc.descriptionUNESP - São Paulo State University Institute of Chemistry Department of Analytical Chemistry Physical Chemistry and Inorganic-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2014/50928-2-
Descrição: dc.descriptionFAPESP: 2015/21412-0-
Descrição: dc.descriptionFAPESP: 2017/16324-0-
Descrição: dc.descriptionCNPq: 465687/2014-8-
Idioma: dc.languageen-
Relação: dc.relationJournal of Drug Delivery Science and Technology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectATR-FTIR-
Palavras-chave: dc.subjectCircular dichroism-
Palavras-chave: dc.subjectDSC-
Palavras-chave: dc.subjectFluorescence-
Palavras-chave: dc.subjectIonic cross-linking-
Palavras-chave: dc.subjectPolyelectrolyte complexation-
Título: dc.titleRational design of nanocarriers based on gellan gum/retrograded starch exploiting polyelectrolyte complexation and ionic cross-linking processes: A potential technological platform for oral delivery of bevacizumab-
Tipo de arquivo: dc.typelivro digital-
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