Role of the YehD fimbriae in the virulence-associated properties of enteroaggregative Escherichia coli

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Virginia School of Medicine-
Autor(es): dc.contributorInstituto Mexicano de Seguro Social-
Autor(es): dc.contributorBenemérita Universidad Autónoma de Puebla-
Autor(es): dc.contributorEmerging Pathogens Institute-
Autor(es): dc.contributorUniversidade Federal do Maranhão-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorInstituto Politécnico Nacional-
Autor(es): dc.contributorInstituto Butantan-
Autor(es): dc.creatorSoria-Bustos, Jorge-
Autor(es): dc.creatorSaitz, Waleska-
Autor(es): dc.creatorMedrano, Abraham-
Autor(es): dc.creatorLara-Ochoa, Cristina-
Autor(es): dc.creatorBennis, Zineb-
Autor(es): dc.creatorMonteiro-Neto, Valerio-
Autor(es): dc.creatordos Santos, Camilla Itapary-
Autor(es): dc.creatorRodrigues, Josias-
Autor(es): dc.creatorHernandes, Rodrigo T.-
Autor(es): dc.creatorYáñez, Jorge A.-
Autor(es): dc.creatorTorres, Javier-
Autor(es): dc.creatorNavarro-García, Fernando-
Autor(es): dc.creatorMartínez-Laguna, Ygnacio-
Autor(es): dc.creatorFontes Piazza, Roxane M.-
Autor(es): dc.creatorMunhoz, Danielle Dias-
Autor(es): dc.creatorCedillo, María L.-
Autor(es): dc.creatorAres, Miguel A.-
Autor(es): dc.creatorDe la Cruz, Miguel A.-
Autor(es): dc.creatorNataro, James P.-
Autor(es): dc.creatorGirón, Jorge A.-
Data de aceite: dc.date.accessioned2025-08-21T22:48:45Z-
Data de disponibilização: dc.date.available2025-08-21T22:48:45Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/1462-2920.15737-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229441-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229441-
Descrição: dc.descriptionThe interaction of enteroaggregative Escherichia coli (EAEC) strains with the colonic gut mucosa is characterized by the ability of the bacteria to form robust biofilms, to bind mucin, and induce a local inflammatory response. These events are mediated by a repertoire of five different aggregative adherence fimbriae variants (AAF/I-V) typically encoded on virulence plasmids. In this study, we report the production in EAEC strains of a new YehD fimbriae (YDF), which is encoded by the chromosomal gene cluster yehABCD, also present in most E. coli strains. Immuno-labelling of EAEC strain 042 with anti-AAF/II and anti-YDF antibodies demonstrated the presence of both AAF/II and YDF on the bacterial surface. We investigated the role of YDF in cell adherence, biofilm formation, colonization of spinach leaves, and induction of pro-inflammatory cytokines release. To this aim, we constructed yehD deletion mutants in different EAEC backgrounds (strains 17-2, 042, 55989, C1010, 278-1, J7) each harbouring one of the five AAFs. The effect of the YDF mutation was strain dependent and AAF independent as the lack of YDF had a different impact on the phenotypes manifested by the different EAECs tested. Expression of the yehABCD operon in a E. coli K12 ORN172 showed that YDF is important for biofilm formation but not for adherence to HeLa cells. Lastly, screening of pro-inflammatory cytokines in supernatants of Caco-2 cells infected with EAEC strains 042 and J7 and their isogenic ΔyehD mutants showed that these mutants were significantly defective in release of IL-8 and TNF-α. This study contributes to the understanding of the complex and diverse mechanisms of adherence of EAEC strains and identifies a new potential target for preventive measures of gastrointestinal illness caused by EAEC and other E. coli pathogroups.-
Descrição: dc.descriptionDepartment of Pediatrics University of Virginia School of Medicine-
Descrição: dc.descriptionUnidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias Centro Médico Nacional Siglo XXI Instituto Mexicano de Seguro Social-
Descrição: dc.descriptionCentro de Detección Biomolecular Benemérita Universidad Autónoma de Puebla-
Descrição: dc.descriptionUniversity of Florida Emerging Pathogens Institute-
Descrição: dc.descriptionDepartamento de Patologia Universidade Federal do Maranhão-
Descrição: dc.descriptionDepartamento de Microbiologia e Imunologia Instituto de Biociencias da UNESP-
Descrição: dc.descriptionFacultad de Estomatología Benemérita Universidad Autónoma de Puebla-
Descrição: dc.descriptionDepartamento de Biología Celular Centro de Investigaciones Avanzadas Instituto Politécnico Nacional-
Descrição: dc.descriptionCentro de Investigaciones en Ciencias Microbiológicas Benemérita Universidad Autónoma de Puebla-
Descrição: dc.descriptionLaboratory of Bacteriology Instituto Butantan-
Descrição: dc.descriptionDepartamento de Microbiologia e Imunologia Instituto de Biociencias da UNESP-
Idioma: dc.languageen-
Relação: dc.relationEnvironmental Microbiology-
???dc.source???: dc.sourceScopus-
Título: dc.titleRole of the YehD fimbriae in the virulence-associated properties of enteroaggregative Escherichia coli-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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