The molecular pathway triggered by zirconia in endothelial cells involves epigenetic control

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Taubaté-
Autor(es): dc.contributorPaulista University-
Autor(es): dc.creatorda C. Fernandes, Célio Júnior-
Autor(es): dc.creatorda Silva, Rodrigo A.-
Autor(es): dc.creatorFretes Wood, Patrícia-
Autor(es): dc.creatorTeixeira, Suélen Aparecida-
Autor(es): dc.creatorBezerra, Fábio-
Autor(es): dc.creatorZambuzzi, Willian F.-
Data de aceite: dc.date.accessioned2025-08-21T21:03:27Z-
Data de disponibilização: dc.date.available2025-08-21T21:03:27Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.tice.2021.101627-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229412-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229412-
Descrição: dc.descriptionThe requirement to achieve natural looking restorations is one of the most challenging aspects in dentistry. Although zirconia has provided new opportunities for achieving superior aesthetics and physicochemical outcomes, very little has been achieved for its cellular and molecular performance, especially considering angiogenesis and osteogenesis. As angiogenesis is a secondary event and concomitant to osteogenesis, an indirect effect of dental implant on endothelial cells could be the release of active molecules such as those already reported affecting osteoblasts. To better address this issue, we challenged human endothelial cells (HUVECs) with zirconia-conditioned medium up to 72 h to allow analysis specific gene expression and protein pattern of mediators of epigenetic machinery in full. Our data shows involvement of zirconia in triggering intracellular signaling through MAPK-ERK activation, leading the signal to activate histone deacetylase HDAC6 likely with concomitant well-modulated DNA methylation profile by DNMTs and TETs. These signaling pathways seem to culminate in cytoskeleton rearrangement of endothelial cells, an important prerequisite to cell migration expected in angiogenesis. Collectively, this study demonstrates for the first time epigenetic-related molecular mechanism involved in endothelial cells responding to zirconia, revealing a repertoire of signaling molecules capable of executing the reprogramming process of gene expression, which are necessary to drive cell proliferation, migration, and consequently angiogenesis. This set of data can further studies using gene editing approaches to better elucidate functional roles.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionLab. of Bioassays and Cellular Dynamics Department of Chemistry and Biochemistry Institute of Biosciences UNESP – São Paulo State University-
Descrição: dc.descriptionDepartment of Dentistry University of Taubaté-
Descrição: dc.descriptionProgram in Environmental and Experimental Pathology Paulista University-
Descrição: dc.descriptionLab. of Bioassays and Cellular Dynamics Department of Chemistry and Biochemistry Institute of Biosciences UNESP – São Paulo State University-
Idioma: dc.languageen-
Relação: dc.relationTissue and Cell-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAngiogenesis-
Palavras-chave: dc.subjectBiomaterial-
Palavras-chave: dc.subjectCell behavior-
Palavras-chave: dc.subjectDental materials-
Palavras-chave: dc.subjectEndothelial-
Palavras-chave: dc.subjectZirconia-
Título: dc.titleThe molecular pathway triggered by zirconia in endothelial cells involves epigenetic control-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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