Cytocompatibility and Synergy of EGCG and Cationic Peptides Against Bacteria Related to Endodontic Infections, in Planktonic and Biofilm Conditions

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorFederal University of Alfenas (UNIFAL-MG)-
Autor(es): dc.creatorCaiaffa, Karina Sampaio-
Autor(es): dc.creatordos Santos, Vanessa Rodrigues-
Autor(es): dc.creatorAbuna, Gabriel Flores-
Autor(es): dc.creatorSantos-Filho, Norival Alves-
Autor(es): dc.creatorCilli, Eduardo Maffud-
Autor(es): dc.creatorSakai, Vivien Thiemy-
Autor(es): dc.creatorCintra, Luciano Tavares Angelo-
Autor(es): dc.creatorDuque, Cristiane-
Data de aceite: dc.date.accessioned2025-08-21T19:52:34Z-
Data de disponibilização: dc.date.available2025-08-21T19:52:34Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s12602-021-09830-3-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229326-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229326-
Descrição: dc.descriptionThis study evaluated the cytocompatibility and antimicrobial/antibiofilm effects of epigallocatechin-3-gallate (EGCG) associated with peptide LL-37 and its analogue KR-12-a5 against oral pathogens. The effect of the compounds on metabolism of fibroblasts was evaluated by methyltetrazolium assays. Antimicrobial activity of the compounds was evaluated on Streptococcus mutans, Enterococcus faecalis, Actinomyces israelii, and Fusobacterium nucleatum under planktonic conditions, on single- and dual-species biofilms and E. faecalis biofilms in dentinal tubules and analyzed by bacterial counts and confocal microscopy. Data were statistically analyzed considering p < 0.05. EGCG and peptide combinations were not toxic to fibroblasts. KR-12-a5 showed synergistic or addictive effects with EGCG and LL-37 against all bacteria tested. However, EGCG associated with KR-12-a5 demonstrated the highest bactericidal activity on all bacteria tested, at lower concentrations. In single-species biofilms, EGCG + KR-12-a5 eliminated S. mutans and A. israelii and reduced E. faecalis and F. nucleatum counts around 5 log CFU/mL. EGCG + KR-12-a5 reduced E. faecalis (-3.93 log CFU/mL) and eliminated S. mutans in dual-species biofilms. No growth of E. faecalis and significant reduction in A. israelii (−6.24 log CFU/mL) and F. nucleatum (−4.62 log CFU/mL) counts were detected in dual-species biofilms. The combination of EGCG and KR-12-a5 led to 88% of E. faecalis dead cells inside dentin tubules. The association of EGCG and KR-12-a5 was cytocompatible and promoted synergistic effect against biofilms of bacteria associated with endodontic infections.-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry Araçatuba Dental School São Paulo State University (UNESP) Aracatuba-
Descrição: dc.descriptionDepartment of Restorative Dentistry Piracicaba Dental School State University of Campinas (UNICAMP) Piracicaba-
Descrição: dc.descriptionDepartment of Biochemistry and Organic Chemistry São Paulo State University - Institute of Chemistry (UNESP) Araraquara-
Descrição: dc.descriptionDepartment of Clinics and Surgery School of Dentistry Federal University of Alfenas (UNIFAL-MG)-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry Araçatuba Dental School São Paulo State University (UNESP) Aracatuba-
Descrição: dc.descriptionDepartment of Biochemistry and Organic Chemistry São Paulo State University - Institute of Chemistry (UNESP) Araraquara-
Idioma: dc.languageen-
Relação: dc.relationProbiotics and Antimicrobial Proteins-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiofilms-
Palavras-chave: dc.subjectCationic antimicrobial peptides-
Palavras-chave: dc.subjectCell culture-
Palavras-chave: dc.subjectEGCG-
Palavras-chave: dc.subjectEndodontics-
Título: dc.titleCytocompatibility and Synergy of EGCG and Cationic Peptides Against Bacteria Related to Endodontic Infections, in Planktonic and Biofilm Conditions-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.