MALDI-TOF MS identification of Prototheca algae associated with bovine mastitis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity of Warsaw-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorFidelis, Carlos E.-
Autor(es): dc.creatorFranke, Manoela-
Autor(es): dc.creatorde Abreu, Letícia C. R.-
Autor(es): dc.creatorJagielski, Tomasz-
Autor(es): dc.creatorRibeiro, Márcio G.-
Autor(es): dc.creatordos Santos, Marcos V.-
Autor(es): dc.creatorGonçalves, Juliano L.-
Data de aceite: dc.date.accessioned2025-08-21T23:36:51Z-
Data de disponibilização: dc.date.available2025-08-21T23:36:51Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1177/10406387211036459-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229296-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229296-
Descrição: dc.descriptionWe evaluated the use of MALDI-TOF MS for the identification of 3 major, dairy-associated Prototheca species, namely, Prototheca bovis (formerly P. zopfii genotype 2), P. blaschkeae, and P. ciferrii (formerly P. zopfii genotype 1). The MALDI-TOF MS spectra established for those species were introduced into the reference spectra library of the Bruker Biotyper MALDI-TOF MS analysis software. Next, 31 Prototheca isolates from Holstein cows with mastitis, from herds located in the midwestern area of São Paulo State, Brazil, were subjected to MALDI-TOF MS profiling. MALDI-TOF MS allowed identification of 22 of 27 P. bovis and 3 of 4 P. blaschkeae isolates with scores >2.0, with 5 of 27 P. bovis and 1 of 4 P. blaschkeae isolates identified only to the genus level. With our extended algae database, MALDI-TOF MS can contribute to quick and effective speciation of Prototheca from mastitis cases.-
Descrição: dc.descriptionDepartment of Animal Nutrition and Production School of Veterinary Medicine and Animal Science University of São Paulo-USP-
Descrição: dc.descriptionDepartment of Medical Microbiology Institute of Microbiology Faculty of Biology University of Warsaw-
Descrição: dc.descriptionDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences São Paulo State University-UNESP-
Descrição: dc.descriptionDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences São Paulo State University-UNESP-
Idioma: dc.languageen-
Relação: dc.relationJournal of Veterinary Diagnostic Investigation-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBrazil-
Palavras-chave: dc.subjectmass spectrometry-
Palavras-chave: dc.subjectmicroalgae-
Palavras-chave: dc.subjectPrototheca-
Título: dc.titleMALDI-TOF MS identification of Prototheca algae associated with bovine mastitis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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