Evaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFaculdade de Tecnologia de Bauru- FATEC-
Autor(es): dc.creatorBarbosa, Adriana Sierra Assencio Almeida-
Autor(es): dc.creatorde Camargo, Mariana Priscila-
Autor(es): dc.creatorde Magalhães Tavares Moreira, Julie Anne Corrêa-
Autor(es): dc.creatorDiman, Rafael Balan-
Autor(es): dc.creatorda Silveira, Mônica-
Data de aceite: dc.date.accessioned2025-08-21T17:02:50Z-
Data de disponibilização: dc.date.available2025-08-21T17:02:50Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-06-02-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.11606/issn.2176-7262.rmrp.2021.166597-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229227-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229227-
Descrição: dc.descriptionIntroduction: The biological safety cabinet (BSC) is a collective protective device used to hold aerosols produced in laboratory procedures. The BSC protects workers, material handling, and the environment. It relies on ultraviolet light (UV) lamps that have germicidal action, altering the nucleic acids of microorganisms. Objective: The objective of the present study was to evaluate the germicidal action of BSC class II type A2 UV light against the culture of two bacterial species with different conditions of exposure to UV light. Methods: For the research, the bacteria Staphylococcus aureus ATCC 25923 and Klebsiella pneumoniae ATCC 10031 were used, at a concentration of 1.5x108 Colony Forming Units/ml (CFU/ml), which were seeded in Petri biplates. Use UV light under different conditions and times. Results: The bacterial species showed the same growth or inhibition profile when subjected to different exposure conditions. Petri dishes with the cover open and protected or not with sterilization packaging showed bacterial inhibition at 15 and 20 minutes. The microorganisms in the biplates protected by the aluminum foil and in the biplates with the cover on, regardless of the time and condition, showed bacterial growth. Conclusion: With the results obtained, it is suggested that the germicidal action of UV light was effective, ensuring adequate decontamination and ensuring quality in laboratory biosafety.-
Descrição: dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho- UNESP-
Descrição: dc.descriptionFaculdade de Tecnologia de Bauru- FATEC, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho- UNESP Botucatu. Hospital Estadual de Bauru, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho- UNESP-
Descrição: dc.descriptionUniversidade Estadual Paulista Júlio de Mesquita Filho- UNESP Botucatu. Hospital Estadual de Bauru, SP-
Idioma: dc.languagept_BR-
Relação: dc.relationMedicina (Brazil)-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiosafety cabinet-
Palavras-chave: dc.subjectKlebsiella pneumoniae-
Palavras-chave: dc.subjectStaphylococcus aureus-
Palavras-chave: dc.subjectUV light-
Título: dc.titleEvaluation of the germicidal action of ultraviolet light in biosafety cabinet concerning bacterial species-
Título: dc.titleAvaliação da ação germicida da luz ultravioleta de cabine de segurança biológica frente às espécies bacterianas-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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