Expression and prognostic significance of vascular endothelial growth factor-A (VEGF-A) and its receptor in canine prostate cancer

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorThe University of Queensland Gatton Campus-
Autor(es): dc.contributorPaulista University—UNIP-
Autor(es): dc.creatorLeis-Filho, Antonio Fernando-
Autor(es): dc.creatorLainetti, Patricia deFaria-
Autor(es): dc.creatorKobayashi, Priscila Emiko-
Autor(es): dc.creatorPalmieri, Chiara-
Autor(es): dc.creatorAmorim, Renée Laufer-
Autor(es): dc.creatorFonseca-Alves, Carlos Eduardo-
Data de aceite: dc.date.accessioned2025-08-21T21:41:35Z-
Data de disponibilização: dc.date.available2025-08-21T21:41:35Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/pros.24199-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229218-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229218-
Descrição: dc.descriptionBackground: Vascular endothelial growth factor-A (VEGF-A) and its receptor, VEGF receptor-2 (VEGFR-2), represent a complex family of angiogenic molecules consisting of different ligands and receptors. Due to the importance of VEGF-A/VEGFR-2 signaling in tumor proliferation and angiogenesis, this study aimed to evaluate the protein and gene expression levels of VEGF-A and VEGFR-2 in canine prostate cancer (PC). Methods: We analyzed VEGF-A and VEGFR-2 expression in 87 PC samples by immunohistochemistry and quantitative-polymerase chain reaction. PC samples were graded according to the Gleason score and the immunohistochemical staining for VEGF-A and VEGFR-2 was quantified using a selected threshold from the ImageJ Software. Microvascular density was assessed by cluster of differentiation 31 staining and counting the number of positive vessels. Additionally, the homology of VEGF-A and VEGFR-2 between humans and dogs was assessed, followed by the construction of a protein structure homology model to compare the tertiary structures of these proteins in both species. Results: Negative to weakly positive expression levels of VEGF-A and VEGFR-2 were observed in the epithelial cells of the normal prostate (NP) and prostatic hyperplasia samples. In contrast, the canine proliferative atrophy and PC samples exhibited higher VEGF-A (p <.0001) and VEGFR-2 (p <.0001) compared to NP. Moreover, positive correlations between the expression levels of VEGF-A and VEGFR-2 (Spearman's coefficient (r) =.68, p =.013) and the expression levels of VEGF-A and VEGFR-2 proteins (r =.8, p <.0001) were also observed in the NP samples. Additionally, the patients with PC exhibiting higher VEGFR-2 expression levels experienced a shorter survival period (p =.0372). Furthermore, we found an association between the microvascular density and overall survival. Dogs with a higher number of vessels showed a shorter survival time. We further demonstrated that the VEGF-A and VEGFR-2 exhibited high homology between humans and dogs, and identified their protein structures in both species. Conclusions: In conclusion, VEGFR-2 appears to be an independent prognostic factor in animals with PC. VEGF-A and VEGFR-2 are highly conserved between humans and dogs, which can be investigated further in future cross-species studies to explore their therapeutic applications.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University—UNESP-
Descrição: dc.descriptionDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University—UNESP-
Descrição: dc.descriptionSchool of Veterinary Science The University of Queensland Gatton Campus-
Descrição: dc.descriptionInstitute of Health Sciences Paulista University—UNIP-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University—UNESP-
Descrição: dc.descriptionDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University—UNESP-
Descrição: dc.descriptionFAPESP: 2015/25400-7-
Formato: dc.format1021-1031-
Idioma: dc.languageen-
Relação: dc.relationProstate-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectangiogenesis-
Palavras-chave: dc.subjectcancer-
Palavras-chave: dc.subjectdog-
Palavras-chave: dc.subjectVEGF-A-
Palavras-chave: dc.subjectVEGFR-2-
Título: dc.titleExpression and prognostic significance of vascular endothelial growth factor-A (VEGF-A) and its receptor in canine prostate cancer-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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