Cattle in vitro induced pluripotent stem cells generated and maintained in 5 or 20% oxygen and different supplementation

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorBessi, Brendon Willian-
Autor(es): dc.creatorBotigelli, Ramon Cesar-
Autor(es): dc.creatorPieri, Naira Caroline Godoy-
Autor(es): dc.creatorMachado, Lucas Simões-
Autor(es): dc.creatorCruz, Jessica Brunhara-
Autor(es): dc.creatorde Moraes, Pamela-
Autor(es): dc.creatorde Souza, Aline Fernanda-
Autor(es): dc.creatorRecchia, Kaiana-
Autor(es): dc.creatorBarbosa, Gabriela-
Autor(es): dc.creatorde Castro, Raquel Vasconcelos Guimarães-
Autor(es): dc.creatorNogueira, Marcelo Fábio Gouveia-
Autor(es): dc.creatorBressan, Fabiana Fernandes-
Data de aceite: dc.date.accessioned2025-08-21T19:48:43Z-
Data de disponibilização: dc.date.available2025-08-21T19:48:43Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/cells10061531-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229144-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229144-
Descrição: dc.descriptionThe event of cellular reprogramming into pluripotency is influenced by several factors, such as in vitro culture conditions (e.g., culture medium and oxygen concentration). Herein, bovine iPSCs (biPSCs) were generated in different levels of oxygen tension (5% or 20% of oxygen) and supple-mentation (bFGF or bFGF + LIF + 2i—bFL2i) to evaluate the efficiency of pluripotency induction and maintenance in vitro. Initial reprogramming was observed in all groups and bFL2i supplementation initially resulted in a superior number of colonies. However, bFL2i supplementation in low oxygen led to a loss of self-renewal and pluripotency maintenance. All clonal lines were positive for alkaline phosphatase; they expressed endogenous pluripotency-related genes SOX2, OCT4 and STELLA. However, expression was decreased throughout the passages without the influence of oxygen tension. GLUT1 and GLUT3 were upregulated by low oxygen. The biPSCs were immunofluorescence-positive stained for OCT4 and SOX2 and they formed embryoid bodies which differentiated in ectoderm and mesoderm (all groups), as well as endoderm (one line from bFL2i in high oxygen). Our study is the first to compare high and low oxygen environments during and after induced reprogramming in cattle. In our conditions, a low oxygen environment did not favor the pluripotency maintenance of biPSCs.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Veterinary Medicine Faculty of Animal Science and Food Engineering University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Pharmacology Institute of Biosciences (IBB) São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Animal Reproduction Faculty of Veterinary Medicine and Animal Sciences University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Pathology Reproduction and One Health Faculty of Agricultural and Veterinary Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biological Science School of Sciences Humanities and Languages São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Pharmacology Institute of Biosciences (IBB) São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Pathology Reproduction and One Health Faculty of Agricultural and Veterinary Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biological Science School of Sciences Humanities and Languages São Paulo State University (UNESP)-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2012/50533-2-
Descrição: dc.descriptionFAPESP: 2015/26818-5-
Descrição: dc.descriptionFAPESP: 2016/16841-2-
Descrição: dc.descriptionFAPESP: 2018/24520-7-
Descrição: dc.descriptionFAPESP: 2019/10751-0-
Descrição: dc.descriptionCAPES: 23038.006964/2014-43-
Descrição: dc.descriptionCNPq: 433133/2018-0-
Idioma: dc.languageen-
Relação: dc.relationCells-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAcquisition of pluripotency-
Palavras-chave: dc.subjectBovine iPSCs-
Palavras-chave: dc.subjectOxygen-
Palavras-chave: dc.subjectPluripotent state-
Título: dc.titleCattle in vitro induced pluripotent stem cells generated and maintained in 5 or 20% oxygen and different supplementation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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