Effect of processing methods on the cytotoxicity of methyl methacrylate-based ocular prostheses: An in vitro study

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Toronto-
Autor(es): dc.creatorda Silva, Emily Vivianne Freitas-
Autor(es): dc.creatorGoiato, Marcelo Coelho-
Autor(es): dc.creatorBitencourt, Sandro Basso-
Autor(es): dc.creatorFiner, Yoav-
Autor(es): dc.creatorBrito, Victor Gustavo Balera-
Autor(es): dc.creatorTakamiya, Aline Satie-
Autor(es): dc.creatorde Oliveira, Sandra Helena Penha-
Autor(es): dc.creatordos Santos, Daniela Micheline-
Data de aceite: dc.date.accessioned2025-08-21T17:10:48Z-
Data de disponibilização: dc.date.available2025-08-21T17:10:48Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.tiv.2021.105211-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229110-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229110-
Descrição: dc.descriptionThe study evaluated the influence of cycles and methods of an ocular prosthesis resin on cytotoxicity toward human conjunctival cells. Resins were polymerized by water bath (WB, 74 °C or 100 °C for 30 min to 9 h), microwave (MW, 1200 W, 3 to 14 min and 30 s at 0 to 720 W), or autopolymerization (AP, room temperature for 20 min ± 60 °C for 30 min). Degree of conversion (DC), cytotoxicity, level of inflammatory mediators, gene expression of different markers, and apoptosis were evaluated. Data were submitted to ANOVA and Tukey test (p < 0.05). WB with longer processing time at higher temperature had highest DC (85.6%) and higher TGF β1-gene expression (1.39); long cycle low power MW showed lowest DC (69.6%), lower cell proliferation (85.4%, MTT), and large IL-2 release (39,297 ng/mL). AP with additional processing time showed lower cell proliferation (75.3%, Alamar Blue), and AP polymerized at room temperature showed higher CASP 9-gene expression (1.21). AP methods showed higher IL-6 release (>277 pg/mL). Short cycle medium power MW had higher IL-23 release (534.2 pg/mL). MW (long and short cycles) and AP polymerizations have triggered a more intense inflammatory response. Among methods recommended by the manufacturer, WB showed high DC and less cytotoxicity.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Aracatuba Dental School Sao Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Aracatuba Dental School UNESP-
Descrição: dc.descriptionOral Oncology Center São Paulo State University (Unesp) School of Dentistry, 1193 José Bonifácio St-
Descrição: dc.descriptionFaculty of Dentistry University of Toronto-
Descrição: dc.descriptionInstitute of Biomedical Engineering University of Toronto-
Descrição: dc.descriptionDepartment of Basic Sciences Aracatuba Dental School UNESP-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Aracatuba Dental School Sao Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Aracatuba Dental School UNESP-
Descrição: dc.descriptionOral Oncology Center São Paulo State University (Unesp) School of Dentistry, 1193 José Bonifácio St-
Descrição: dc.descriptionDepartment of Basic Sciences Aracatuba Dental School UNESP-
Idioma: dc.languageen-
Relação: dc.relationToxicology in Vitro-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCellular immune response-
Palavras-chave: dc.subjectCytotoxicity-
Palavras-chave: dc.subjectMethyl methacrylate-
Palavras-chave: dc.subjectOcular prosthesis-
Título: dc.titleEffect of processing methods on the cytotoxicity of methyl methacrylate-based ocular prostheses: An in vitro study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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