The recombinant plant Bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters

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Autor(es): dc.contributorUniversidade Federal de São Paulo (UNIFESP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorOliveira, Cleide-
Autor(es): dc.creatorValois, Mayara Vioto-
Autor(es): dc.creatorOttaiano, Tatiana Fontes-
Autor(es): dc.creatorMiranda, Antonio-
Autor(es): dc.creatorHansen, Daiane-
Autor(es): dc.creatorSampaio, Misako Uemura-
Autor(es): dc.creatorOliva, Maria Luiza Vilela-
Autor(es): dc.creatorde Abreu Maffei, Francisco Humberto-
Data de aceite: dc.date.accessioned2025-08-21T23:28:01Z-
Data de disponibilização: dc.date.available2025-08-21T23:28:01Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-021-92745-4-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229069-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229069-
Descrição: dc.descriptionThe anti-inflammatory effects of the plant protease inhibitor BbCI (Bauhinia bauhinioides cruzipain inhibitor), which blocks elastase, cathepsin G, and L, and proteinase 3 has been demonstrated. Here, we investigated the recombinant rBbCI-His(6) (containing a histidine tail) in an experimental venous thrombosis model of vena cava (VC) ligature in rats, comparing to heparin. We evaluate the effects of the inhibitors (native or recombinant) or heparin on the activated partial thromboplastin time (aPTT) and prothrombin time (PT) in human and rat plasmas. The rats undergoing treatment received a saline solution or increasing concentrations of rBbCI-His(6), heparin, or a mixture of both. After 4 h of ligature VC, thrombus, if present was removed and weighed. aPTT, PT, and cytokines were measured in blood collected by cardiac puncture. aPTT, PT, and bleeding time (BT) were also measured at the time of VC (vena cava) ligature. rBbCI-His(6) (0.45 or 1.40 mg/kg) does not alter aPTT, PT or BT. No differences in coagulation parameters were detected in rBbCI-His(6) treated rats at the time of VC ligature or when the thrombus was removed. There was a significant decrease in the weight of thrombus in the animals of the groups treated with the rBbCI-His(6) (1.40 mg/kg), with the rBbCI-His(6) mixture (1.40 mg/kg) + heparin (50 IU/kg) and heparin (100 IU/kg) in relation to control group (saline). The growth-related oncogene/keratinocyte chemoattractant (GRO/KC) serum levels in rats treated with rBbCI-His(6) (1.40 mg/kg) or heparin (200 IU/kg) were reduced. In the experimental model used, rBbCI-His(6) alone had an antithrombotic effect, not altering blood clotting or bleeding time.-
Descrição: dc.descriptionDepartamento de Bioquímica Universidade Federal de São Paulo, Rua Três de Maio, 100-
Descrição: dc.descriptionDepartamento de Biofísica Universidade Federal de São Paulo-
Descrição: dc.descriptionDepartamento de Cirurgia e Ortopedia Universidade Estadual Paulista-
Descrição: dc.descriptionDepartamento de Cirurgia e Ortopedia Universidade Estadual Paulista-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Título: dc.titleThe recombinant plant Bauhinia bauhinioides elastase inhibitor reduces rat thrombus without alterations in hemostatic parameters-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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