Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorBrazilian Ministry of Health-
Autor(es): dc.contributorOswaldo Cruz Foundation-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorVieira de Araujo, Anna Erika-
Autor(es): dc.creatorConde, Luis Vidal-
Autor(es): dc.creatorda Silva Junior, Haroldo Cid-
Autor(es): dc.creatorde Almeida Machado, Lucas-
Autor(es): dc.creatorLara, Flavio Alves-
Autor(es): dc.creatorChapeaurouge, Alex-
Autor(es): dc.creatorPauer, Heidi-
Autor(es): dc.creatorPires Hardoim, Cristiane Cassiolato-
Autor(es): dc.creatorMartha Antunes, Luis Caetano-
Autor(es): dc.creatorD'Alincourt Carvalho-Assef, Ana Paula-
Autor(es): dc.creatorMoreno Senna, Jose Procopio-
Data de aceite: dc.date.accessioned2025-08-21T22:29:45Z-
Data de disponibilização: dc.date.available2025-08-21T22:29:45Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.micinf.2021.104801-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229067-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229067-
Descrição: dc.descriptionAcinetobacter baumannii is an important nosocomial pathogen. BamA is a protein that belongs to a complex responsible for organizing the proteins on the bacterial outer membrane. In this work, we aimed to evaluate murine immune responses to BamA recombinant protein (rAbBamA) from A. baumannii in an animal model of infection, and to assess cross-reactivity of this target for the development of anti-A. baumannii vaccines or diagnostics. Immunization of mice with rAbBamA elicited high antibody titers and antibody recognition of native A. baumannii BamA. Immunofluorescence also detected binding to the bacterial surface. After challenge, immunized mice demonstrated a 40% survival increase and better bacterial clearance in kidneys. Immunoblot of anti-rAbBamA against other medically relevant bacteria showed binding to proteins of approximately 35 kDa in Klebsiella pneumoniae and Escherichia coli lysates, primarily identified as OmpA and OmpC, respectively. Altogether, our data show that anti-rAbBamA antibodies provide a protective response against A. baumannii infection in mice. However, the response elicited by immunization with rAbBamA is not completely specific to A. baumannii. Although a broad-spectrum vaccine that protects against various pathogens is an appealing strategy, antibody reactivity against the human microbiota is undesired. In fact, immunization with rAbBamA produced noticeable effects on the gut microbiota. However, the changes elicited were small and non-specific, given that no significant changes in the abundance of Proteobacteria were observed. Overall, rAbBamA is a promising target, but specificity must be considered in the development of immunological tools against A. baumannii.-
Descrição: dc.descriptionBio-Manguinhos Oswaldo Cruz Foundation Brazilian Ministry of Health-
Descrição: dc.descriptionOswaldo Cruz Institute Oswaldo Cruz Foundation Brazilian Ministry of Health-
Descrição: dc.descriptionNational Institute of Science and Technology of Innovation on Diseases of Neglected Populations Center for Technological Development in Health Oswaldo Cruz Foundation-
Descrição: dc.descriptionSão Paulo State University Institute of Biosciences Coastal Campus of São Vicente-
Descrição: dc.descriptionSão Paulo State University Institute of Biosciences Coastal Campus of São Vicente-
Idioma: dc.languageen-
Relação: dc.relationMicrobes and Infection-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAcinetobacter baumannii-
Palavras-chave: dc.subjectBamA-
Palavras-chave: dc.subjectImmunogenic-
Palavras-chave: dc.subjectResistant bacteria-
Palavras-chave: dc.subjectSpecificity-
Título: dc.titleCross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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