New Multiscale Characterization Methodology for Effective Determination of Isolation–Structure–Function Relationship of Extracellular Vesicles

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Autor(es): dc.contributorThe University of Sydney-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorNational Measurement Institute Australia-
Autor(es): dc.contributorThe University of Melbourne-
Autor(es): dc.contributorHarvard T.H. Chan School of Public Health-
Autor(es): dc.contributorInstitute for Basic Science (IBS)-
Autor(es): dc.contributorUlsan National Institute of Science and Technology (UNIST)-
Autor(es): dc.creatorPhan, Thanh Huyen-
Autor(es): dc.creatorDivakarla, Shiva Kamini-
Autor(es): dc.creatorYeo, Jia Hao-
Autor(es): dc.creatorLei, Qingyu-
Autor(es): dc.creatorTharkar, Priyanka-
Autor(es): dc.creatorPansani, Taisa Nogueira-
Autor(es): dc.creatorLeslie, Kathryn G.-
Autor(es): dc.creatorTong, Maggie-
Autor(es): dc.creatorColeman, Victoria A.-
Autor(es): dc.creatorJämting, Åsa-
Autor(es): dc.creatorDu Plessis, Mar-Dean-
Autor(es): dc.creatorNew, Elizabeth J.-
Autor(es): dc.creatorKalionis, Bill-
Autor(es): dc.creatorDemokritou, Philip-
Autor(es): dc.creatorWoo, Hyun-Kyung-
Autor(es): dc.creatorCho, Yoon-Kyoung-
Autor(es): dc.creatorChrzanowski, Wojciech-
Data de aceite: dc.date.accessioned2025-08-21T18:23:17Z-
Data de disponibilização: dc.date.available2025-08-21T18:23:17Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-06-07-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fbioe.2021.669537-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/229015-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/229015-
Descrição: dc.descriptionExtracellular vesicles (EVs) have been lauded as next-generation medicines, but very few EV-based therapeutics have progressed to clinical use. Limited clinical translation is largely due to technical barriers that hamper our ability to mass produce EVs, i.e., to isolate, purify, and characterize them effectively. Technical limitations in comprehensive characterization of EVs lead to unpredicted biological effects of EVs. Here, using a range of optical and non-optical techniques, we showed that the differences in molecular composition of EVs isolated using two isolation methods correlated with the differences in their biological function. Our results demonstrated that the isolation method determines the composition of isolated EVs at single and sub-population levels. Besides the composition, we measured for the first time the dry mass and predicted sedimentation of EVs. These parameters were likely to contribute to the biological and functional effects of EVs on single cell and cell cultures. We anticipate that our new multiscale characterization approach, which goes beyond traditional experimental methodology, will support fundamental understanding of EVs as well as elucidate the functional effects of EVs in in vitro and in vivo studies. Our findings and methodology will be pivotal for developing optimal isolation methods and establishing EVs as mainstream therapeutics and diagnostics. This innovative approach is applicable to a wide range of sectors including biopharma and biotechnology as well as to regulatory agencies.-
Descrição: dc.descriptionMedical Advances Without Animals Trust-
Descrição: dc.descriptionSydney School of Pharmacy Faculty of Medicine and Health Sydney Nano Institute The University of Sydney-
Descrição: dc.descriptionSchool of Chemistry The University of Sydney-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry UNESP-Universidade Estadual Paulista-
Descrição: dc.descriptionNanometrology Section National Measurement Institute Australia-
Descrição: dc.descriptionSchool of Chemistry Faculty of Science Sydney Nano Institute The University of Sydney-
Descrição: dc.descriptionMaternal-Fetal Medicine Pregnancy Research Centre The Royal Women’s Hospital Department of Obstetrics and Gynaecology The University of Melbourne-
Descrição: dc.descriptionDepartment of Environmental Health Center for Nanotechnology and Nanotoxicology Harvard T.H. Chan School of Public Health-
Descrição: dc.descriptionCenter for Soft and Living Matter Institute for Basic Science (IBS)-
Descrição: dc.descriptionDepartment of Biomedical Engineering Ulsan National Institute of Science and Technology (UNIST)-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry UNESP-Universidade Estadual Paulista-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Bioengineering and Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectexosomes-
Palavras-chave: dc.subjectextracellular vesicles-
Palavras-chave: dc.subjectisolation methods-
Palavras-chave: dc.subjectmesenchymal stromal/stem cell-
Palavras-chave: dc.subjectnanodosimetry-
Palavras-chave: dc.subjectsingle vesicle analysis-
Palavras-chave: dc.subjecttangential flow filtration-
Palavras-chave: dc.subjectultracentrifugation-
Título: dc.titleNew Multiscale Characterization Methodology for Effective Determination of Isolation–Structure–Function Relationship of Extracellular Vesicles-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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