Successful application of ancient DNA extraction and library construction protocols to museum wet collection specimens

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity Museum of Bergen-
Autor(es): dc.contributorSNSB Bavarian State Collection of Zoology-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorBraunschweig University of Technology-
Autor(es): dc.contributorUniversity of Potsdam-
Autor(es): dc.contributorMuseum für Naturkunde– Leibniz Institute for Evolution and Biodiversity Science-
Autor(es): dc.contributorAlbert Ludwigs University Freiburg-
Autor(es): dc.contributorUniversity of Copenhagen-
Autor(es): dc.contributorCambridge University-
Autor(es): dc.contributorRega Institute for Medical Research-
Autor(es): dc.contributorNottingham Trent University-
Autor(es): dc.creatorStraube, Nicolas-
Autor(es): dc.creatorLyra, Mariana L.-
Autor(es): dc.creatorPaijmans, Johanna L. A.-
Autor(es): dc.creatorPreick, Michaela-
Autor(es): dc.creatorBasler, Nikolas-
Autor(es): dc.creatorPenner, Johannes-
Autor(es): dc.creatorRödel, Mark-Oliver-
Autor(es): dc.creatorWestbury, Michael V.-
Autor(es): dc.creatorHaddad, Célio F. B.-
Autor(es): dc.creatorBarlow, Axel-
Autor(es): dc.creatorHofreiter, Michael-
Data de aceite: dc.date.accessioned2025-08-21T18:00:57Z-
Data de disponibilização: dc.date.available2025-08-21T18:00:57Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2021-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/1755-0998.13433-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/228984-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/228984-
Descrição: dc.descriptionMillions of scientific specimens are housed in museum collections, a large part of which are fluid preserved. The use of formaldehyde as fixative and subsequent storage in ethanol is especially common in ichthyology and herpetology. This type of preservation damages DNA and reduces the chance of successful retrieval of genetic data. We applied ancient DNA extraction and single stranded library construction protocols to a variety of vertebrate samples obtained from wet collections and of different ages. Our results show that almost all samples tested yielded endogenous DNA. Archival DNA extraction was successful across different tissue types as well as using small amounts of tissue. Conversion of archival DNA fragments into single-stranded libraries resulted in usable data even for samples with initially undetectable DNA amounts. Subsequent target capture approaches for mitochondrial DNA using homemade baits on a subset of 30 samples resulted in almost complete mitochondrial genome sequences in several instances. Thus, application of ancient DNA methodology makes wet collection specimens, including type material as well as rare, old or extinct species, accessible for genetic and genomic analyses. Our results, accompanied by detailed step-by-step protocols, are a large step forward to open the DNA archive of museum wet collections for scientific studies.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDeutsche Forschungsgemeinschaft-
Descrição: dc.descriptionUniversity Museum of Bergen-
Descrição: dc.descriptionSNSB Bavarian State Collection of Zoology-
Descrição: dc.descriptionDepartamento de Biodiversidade Instituto de Biociências and Centro de Aquicultura (CAUNESP) Laboratório de Herpetologia Universidade Estadual Paulista - UNESP-
Descrição: dc.descriptionZoological Institute Braunschweig University of Technology-
Descrição: dc.descriptionDepartment of Mathematics and Natural Sciences Evolutionary Adaptive Genomics Institute for Biochemistry and Biology University of Potsdam-
Descrição: dc.descriptionMuseum für Naturkunde– Leibniz Institute for Evolution and Biodiversity Science-
Descrição: dc.descriptionChair of Wildlife Ecology and Management Albert Ludwigs University Freiburg-
Descrição: dc.descriptionSection for Evolutionary Genomics The GLOBE Institute University of Copenhagen-
Descrição: dc.descriptionDepartment of Zoology Cambridge University-
Descrição: dc.descriptionDepartment of Microbiology Immunology and Transplantation Division of Clinical and Epidemiological Virology Rega Institute for Medical Research-
Descrição: dc.descriptionSchool of Science and Technology Nottingham Trent University-
Descrição: dc.descriptionDepartamento de Biodiversidade Instituto de Biociências and Centro de Aquicultura (CAUNESP) Laboratório de Herpetologia Universidade Estadual Paulista - UNESP-
Descrição: dc.descriptionFAPESP: #2013/50741-7-
Descrição: dc.descriptionFAPESP: #2017/2616-8-
Descrição: dc.descriptionFAPESP: #2018/15425-0-
Descrição: dc.descriptionCNPq: 306623/2018-8-
Descrição: dc.descriptionDeutsche Forschungsgemeinschaft: 351649567-
Descrição: dc.descriptionCNPq: 431589/2016-0-
Formato: dc.format2299-2315-
Idioma: dc.languageen-
Relação: dc.relationMolecular Ecology Resources-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectancient DNA-
Palavras-chave: dc.subjectarchival DNA-
Palavras-chave: dc.subjectbiological collection-
Palavras-chave: dc.subjectformalin-
Palavras-chave: dc.subjectsingle-stranded DNA library-
Palavras-chave: dc.subjecttarget capture-
Título: dc.titleSuccessful application of ancient DNA extraction and library construction protocols to museum wet collection specimens-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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