In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorItaquera-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade do Vale do Paraiba-
Autor(es): dc.contributorUFPI-Universidade Federal do Piauí-
Autor(es): dc.creatorAndrade, Telmo M.-
Autor(es): dc.creatorMello, Daphne C. R.-
Autor(es): dc.creatorElias, Conceição M. V.-
Autor(es): dc.creatorAbdala, Julia M. A.-
Autor(es): dc.creatorSilva, Edmundo-
Autor(es): dc.creatorVasconcellos, Luana M. R.-
Autor(es): dc.creatorTim, Carla R.-
Autor(es): dc.creatorMarciano, Fernanda R.-
Autor(es): dc.creatorLobo, Anderson O.-
Data de aceite: dc.date.accessioned2025-08-21T16:07:22Z-
Data de disponibilização: dc.date.available2025-08-21T16:07:22Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2019-02-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10856-019-6222-1-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/228658-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/228658-
Descrição: dc.descriptionHerein, poly(ɛ-caprolactone) (PCL) mats with different amounts of nanohydroxyapatite (nHAp) were produced using rotary-jet spinning (RJS) and evaluated in vitro and in vivo. The mean fiber diameters of the PCL, PCL/nHAp (3%), PCL/nHAp (5%), and PCL/nHAp (20%) scaffolds were 1847 ± 1039, 1817 ± 1044, 1294 ± 4274, and 845 ± 248 nm, respectively. Initially, all the scaffolds showed superhydrophobic behavior (contact angle around of 140 o C), but decreased to 80° after 30 min. All the produced scaffolds were bioactive after soaking in simulated body fluid, especially PCL/nHAp (20%). The crystallinity of the PCL scaffolds decreased progressively from 46 to 21% after incorporation of 20% nHAp. In vitro and in vivo cytotoxicity were investigated, as well as the mats’ ability to reduce bacteria biofilm formation. In vitro cellular differentiation was evaluated by measuring alkaline phosphatase activity and mineralized nodule formation. Overall, we identified the total ideal amount of nHAp to incorporate in PCL mats, which did not show in vitro or in vivo cytotoxicity and promoted lamellar bone formation independently of the amounts of nHAp. The scaffolds with nHAp showed reduced bacterial proliferation. Alizarin red staining was higher in materials associated with nHAp than in those without nHAp. Overall, this study demonstrates that PCL with nHAp prepared by RJS merits further evaluation for orthopedic applications.[Figure not available: see fulltext.]-
Descrição: dc.descriptionInstituto Científico e Tecnológico Universidade Brasil Itaquera-
Descrição: dc.descriptionDepartamento de Biociência e Diagnóstico Oral Instituto de Ciência e Tecnologia Universidade Estadual de São Paulo, São Jose dos Campos-
Descrição: dc.descriptionInstituto de Pesquisa e Desenvolvimento Universidade do Vale do Paraiba, São Jose dos Campos-
Descrição: dc.descriptionLIMAV-Laboratório Interdisciplinar de Materiais Avançados PPGCM-Programa de Pós-graduação em Ciência e Engenharia de Materiais UFPI-Universidade Federal do Piauí-
Descrição: dc.descriptionDepartamento de Biociência e Diagnóstico Oral Instituto de Ciência e Tecnologia Universidade Estadual de São Paulo, São Jose dos Campos-
Idioma: dc.languageen-
Relação: dc.relationJournal of Materials Science: Materials in Medicine-
???dc.source???: dc.sourceScopus-
Título: dc.titleIn vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite-
Tipo de arquivo: dc.typelivro digital-
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