DC-STAMP Is an Osteoclast Fusogen Engaged in Periodontal Bone Resorption

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Autor(es): dc.contributorChulalongkorn University-
Autor(es): dc.contributorForsyth Institute-
Autor(es): dc.contributorHarvard School of Dental Medicine-
Autor(es): dc.contributorPeriodontal Medicine-
Autor(es): dc.contributorTokyo Dental College-
Autor(es): dc.contributorOhio State University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorResearch and Development Headquarters-
Autor(es): dc.contributorUCLA-
Autor(es): dc.contributorCollege of Dental Medicine-
Autor(es): dc.creatorWisitrasameewong, W.-
Autor(es): dc.creatorKajiya, M.-
Autor(es): dc.creatorMovila, A.-
Autor(es): dc.creatorRittling, S.-
Autor(es): dc.creatorIshii, T.-
Autor(es): dc.creatorSuzuki, M.-
Autor(es): dc.creatorMatsuda, S.-
Autor(es): dc.creatorMazda, Y.-
Autor(es): dc.creatorTorruella, M. R.-
Autor(es): dc.creatorAzuma, M. M.-
Autor(es): dc.creatorEgashira, K.-
Autor(es): dc.creatorFreire, M. O.-
Autor(es): dc.creatorSasaki, H.-
Autor(es): dc.creatorWang, C. Y.-
Autor(es): dc.creatorHan, X.-
Autor(es): dc.creatorTaubman, M. A.-
Autor(es): dc.creatorKawai, T.-
Data de aceite: dc.date.accessioned2025-08-21T15:31:08Z-
Data de disponibilização: dc.date.available2025-08-21T15:31:08Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2017-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1177/0022034517690490-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/228328-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/228328-
Descrição: dc.descriptionDendritic cell-specific transmembrane protein (DC-STAMP) plays a key role in the induction of osteoclast (OC) cell fusion, as well as DC-mediated immune regulation. While DC-STAMP gene expression is upregulated in the gingival tissue with periodontitis, its pathophysiological roles in periodontitis remain unclear. To evaluate the effects of DC-STAMP in periodontitis, anti-DC-STAMP-monoclonal antibody (mAb) was tested in a mouse model of ligature-induced periodontitis (n = 6-7/group) where Pasteurella pneumotropica (Pp)-reactive immune response activated T cells to produce receptor activator of nuclear factor kappa-B ligand (RANKL), which, in turn, promotes the periodontal bone loss via upregulation of osteoclastogenesis. DC-STAMP was expressed on the cell surface of mature multinuclear OCs, as well as immature mononuclear OCs, in primary cultures of RANKL-stimulated bone marrow cells. Anti-DC-STAMP-mAb suppressed the emergence of large, but not small, multinuclear OCs, suggesting that DC-STAMP is engaged in the late stage of cell fusion. Anti-DC-STAMP-mAb also inhibited pit formation caused by RANKL-stimulated bone marrow cells. Attachment of ligature to a second maxillary molar induced DC-STAMP messenger RNA and protein, along with elevated tartrate-resistant acid phosphatase-positive (TRAP+) OCs and alveolar bone loss. As we expected, systemic administration of anti-DC-STAMP-mAb downregulated the ligature-induced alveolar bone loss. Importantly, local injection of anti-DC-STAMP-mAb also suppressed alveolar bone loss and reduced the total number of multinucleated TRAP+ cells in mice that received ligature attachment. Attachment of ligature induced significantly elevated tumor necrosis factor-α, interleukin-1β, and RANKL in the gingival tissue compared with the control site without ligature (P < 0.05), which was unaffected by local injection with either anti-DC-STAMP-mAb or control-mAb. Neither in vivo anti-Pp IgG antibody nor in vitro anti-Pp T-cell response and resultant production of RANKL was affected by anti-DC-STAMP-mAb. This study illustrated the roles of DC-STAMP in promoting local OC cell fusion without affecting adaptive immune responses to oral bacteria. Therefore, it is plausible that a novel therapeutic regimen targeting DC-STAMP could suppress periodontal bone loss.-
Descrição: dc.descriptionDepartment of Periodontology Faculty of Dentistry Chulalongkorn University-
Descrição: dc.descriptionDepartment of Immunology and Infectious Diseases Forsyth Institute-
Descrição: dc.descriptionHarvard School of Dental Medicine-
Descrição: dc.descriptionHiroshima University Graduate School of Biomedical Sciences Periodontal Medicine-
Descrição: dc.descriptionTokyo Dental College-
Descrição: dc.descriptionCollege of Dentistry Ohio State University-
Descrição: dc.descriptionAraçatuba Dental School Department of Endodontics UnivEstadual Paulista-
Descrição: dc.descriptionLION Corporation Research and Development Headquarters-
Descrição: dc.descriptionUCLA Lab of Molecular Signaling Division of Oral Biology and Medicine UCLA-
Descrição: dc.descriptionDepartment of Periodontology NOVA Southeastern University College of Dental Medicine, 3200 South University Drive-
Descrição: dc.descriptionAraçatuba Dental School Department of Endodontics UnivEstadual Paulista-
Formato: dc.format685-693-
Idioma: dc.languageen-
Relação: dc.relationJournal of Dental Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbone resorption-
Palavras-chave: dc.subjectcell fusion-
Palavras-chave: dc.subjectimmunity-
Palavras-chave: dc.subjectmice-
Palavras-chave: dc.subjectosteoclasts-
Palavras-chave: dc.subjectperiodontal disease(s)/periodontitis-
Título: dc.titleDC-STAMP Is an Osteoclast Fusogen Engaged in Periodontal Bone Resorption-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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