Screening of endophytic sources of exopolysaccharides: Preliminary characterization of crude exopolysaccharide produced by submerged culture of Diaporthe sp. JF766998 under different cultivation time

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Maringá (UEM)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorOrlandelli, Ravely Casarotti-
Autor(es): dc.creatorVasconcelos, Ana Flora Dalberto-
Autor(es): dc.creatorAzevedo, João Lúcio-
Autor(es): dc.creatorCorradi Da Silva, Maria De Lourdes-
Autor(es): dc.creatorPamphile, João Alencar-
Data de aceite: dc.date.accessioned2025-08-21T17:23:32Z-
Data de disponibilização: dc.date.available2025-08-21T17:23:32Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2016-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.biopen.2016.02.003-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/228132-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/228132-
Descrição: dc.descriptionEndophytic fungi have been described as producers of important bioactive compounds; however, they remain under-exploited as exopolysaccharides (EPS) sources. Therefore, this work reports on EPS production by submerged cultures of eight endophytes isolated from Piper hispidum Sw.; belonging to genera Diaporthe, Marasmius, Phlebia, Phoma, Phyllosticta and Schizophyllum. After fermentation for 96 h, four endophytes secreted EPS: Diaporthe sp. JF767000, Diaporthe sp. JF766998, Diaporthe sp. JF767007 and Phoma herbarum JF766995. The EPS from Diaporthe sp. JF766998 differed statistically from the others, with a higher percentage of carbohydrate (91%) and lower amount of protein (8%). Subsequently, this fungus was grown under submerged culture for 72, 96 and 168 h (these EPS were designated EPSD1-72, EPSD1-96 and EPSD1-168) and the differences in production, monosaccharide composition and apparent molecular were compared. The EPS yields in mg/100 mL of culture medium were: 3.0 ± 0.4 (EPSD1-72), 15.4 ± 2.2 (EPSD1-96) and 14.8 ± 1.8 (EPSD1-168). The EPSD1-72 had high protein content (28.5%) and only 71% of carbohydrate; while EPSD1-96 and EPSD1-168 were composed mainly of carbohydrate (∼95 and 100%, respectively), with low protein content (∼5%) detected at 96 h. Galactose was the main monosaccharide component (30%) of EPSD1-168. Differently, EPSD1-96 was rich in glucose (51%), with molecular weight of 46.6 kDa. It is an important feature for future investigations, because glucan-rich EPS are reported as effective antitumor agents.-
Descrição: dc.descriptionUniversidade Estadual de Maringá Departamento de Biotecnologia Genética e Biologia Celular-
Descrição: dc.descriptionUniversidade Estadual Paulista Faculdade de Ciências e Tecnologia Departamento de Química e Bioquímica-
Descrição: dc.descriptionUniversidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz-
Descrição: dc.descriptionUniversidade Estadual Paulista Faculdade de Ciências e Tecnologia Departamento de Química e Bioquímica-
Formato: dc.format33-40-
Idioma: dc.languageen-
Relação: dc.relationBiochimie Open-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEndophytes-
Palavras-chave: dc.subjectEPS-
Palavras-chave: dc.subjectHPSEC/RID-
Palavras-chave: dc.subjectMonosaccharide composition-
Título: dc.titleScreening of endophytic sources of exopolysaccharides: Preliminary characterization of crude exopolysaccharide produced by submerged culture of Diaporthe sp. JF766998 under different cultivation time-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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