Evaluation of recovery, quality and In Vitro nuclear maturation of oocytes obtained from buffalo and bovine ovaries

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorLeal, L. S.-
Autor(es): dc.creatorMoya-Araújo, C. F.-
Autor(es): dc.creatorFernandes, C. B.-
Autor(es): dc.creatorMartins, L. R.-
Autor(es): dc.creatorLandim-Alvarenga, F. C.-
Autor(es): dc.creatorOba, E.-
Data de aceite: dc.date.accessioned2025-08-21T20:19:29Z-
Data de disponibilização: dc.date.available2025-08-21T20:19:29Z-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2022-04-29-
Data de envio: dc.date.issued2010-01-01-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/227833-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/227833-
Descrição: dc.descriptionOvaries of 86 buffaloes and 95 cows were collected from slaughterhouses and transported to the laboratory in saline solution at 36°C. The Cumulus-oocyte complexes (COCs) were recovered by follicular aspiration and only grades I and II COCs were selected and matured in TCM 199 supplemented with 10% fetal calf serum, sodium pyruvate, LH, FSH, estradiol, gentamicin and cysteamin, for 22-24 hours. A total of 714 and 1983 COCs were recovered from buffaloes and cows, respectively. In the buffaloes, the recovery rates of each COCs categories (grade I: 25.9%; grade II: 30.7%; grade III: 10.2%; denuded 18.6% and expanded: 14.6%) were lower than cows (31.8; 30.6; 15.4; 4.5; 17.7%, respectively) according to Mann-Whitney Test (p< 0.05). The percentage of bubaline oocytes that reached metaphase II (63.4% - 242/396) was lower than bovine oocytes (67.8% - 696/1234) under the same laboratory conditions. These differences observed in all analysis realized indicate that each species has peculiar phisiological characteristics.-
Descrição: dc.descriptionDepartment of Animal Reproduction and Veterinary Radiology, College of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), 18618-000 -Botucatu, São Paulo State-
Descrição: dc.descriptionDepartment of Animal Reproduction and Veterinary Radiology, College of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), 18618-000 -Botucatu, São Paulo State-
Formato: dc.format892-894-
Idioma: dc.languageen-
Relação: dc.relationRevista Veterinaria-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBuffaloes-
Palavras-chave: dc.subjectCows-
Palavras-chave: dc.subjectIn vitro maturation-
Palavras-chave: dc.subjectOocytes-
Título: dc.titleEvaluation of recovery, quality and In Vitro nuclear maturation of oocytes obtained from buffalo and bovine ovaries-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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