Diagnosis of Giardia infections by PCR-based methods in children of an endemic area

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUSC-
Autor(es): dc.creatorDavid, E. B.-
Autor(es): dc.creatorCoradi, S. T.-
Autor(es): dc.creatorOliveira-Sequeira, T. C.G.-
Autor(es): dc.creatorRibolla, P. E.M.-
Autor(es): dc.creatorKatagiri, S.-
Autor(es): dc.creatorGuimarães, S.-
Data de aceite: dc.date.accessioned2025-08-21T18:32:57Z-
Data de disponibilização: dc.date.available2025-08-21T18:32:57Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2011-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/s1678-91992011000200012-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/226362-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/226362-
Descrição: dc.descriptionThe present study was designed to estimate the prevalence of Giardia infection in preschool- and school-aged children living in an endemic area. Fecal samples from 573 children were processed by zinc sulfate centrifugal flotation, centrifugal sedimentation (using a commercial device for fecal concentration - TF-Test kit®) and polymerase chain reaction (PCR)-based methods. Of the stool samples assessed, 277 (48.3%) were positive for intestinal parasites and/or commensal protozoa. Centrifugal flotation presented the highest diagnostic sensitivity for Giardia infections. The kappa index revealed that both coproparasitological techniques closely agreed on the Giardia diagnosis (86%) versus satisfactory (72%) and poor (35%) concordances for commensal protozoan and helminth infections, respectively. Concerning Giardia molecular diagnosis, from the 71 microscopy-positive samples, specific amplification of gdh and tpi fragments was noted in 68 (95.7%) and 64 (90%) samples, respectively. Amplification of gdh and tpi genes was observed, respectively, in 95.7% and 90% of microscopy-positive Giardia samples. For 144 microscopy-negative samples, gdh and tpi gene amplification products were obtained from 8.3% and 35.9% samples, respectively. The agreement between these genes was about 40%. The centrifuge-flotation based method was the most suitable means of Giardia diagnosis assessed in the present study by combining accuracy and low cost. © CEVAP 2011.-
Descrição: dc.descriptionDepartment of Parasitology Botucatu Biosciences Institute São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, São Paulo State-
Descrição: dc.descriptionDepartment of Biological and Health Sciences Sacred Heart University USC, Bauru, São Paulo State-
Descrição: dc.descriptionDepartment of Parasitology Botucatu Biosciences Institute São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, São Paulo State-
Formato: dc.format209-215-
Idioma: dc.languageen-
Relação: dc.relationJournal of Venomous Animals and Toxins Including Tropical Diseases-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectChildren-
Palavras-chave: dc.subjectDiagnosis-
Palavras-chave: dc.subjectGiardia duodenalis-
Palavras-chave: dc.subjectPCR-
Título: dc.titleDiagnosis of Giardia infections by PCR-based methods in children of an endemic area-
Tipo de arquivo: dc.typelivro digital-
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