Effect of exogenous galectin-1 on leukocyte migration: Modulation of cytokine levels and adhesion molecules

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorSão José do Rio Preto School of Medicine - FAMERP-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorGil, Cristiane D.-
Autor(es): dc.creatorGullo, Caio E.-
Autor(es): dc.creatorOliani, Sonia M.-
Data de aceite: dc.date.accessioned2025-08-21T20:41:44Z-
Data de disponibilização: dc.date.available2025-08-21T20:41:44Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2011-11-02-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/226306-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/226306-
Descrição: dc.descriptionThe effect of exogenous Gal-1 on cellular response and adhesion molecule expression was investigated in a classical model of acute inflammation induced by zymosan. C57BL6 mice, treated or not with human recombinant (hr) Gal-1, received i.p. injection of zymosan and peritoneal exudate, blood and mesentery were processed for cellular, biochemical, light and electron microscopic analysis after 4 and 24 h. Zymosan peritonitis provoked the expected signs of inflammation at 4 h, including a significant increase in extravasated PMNs in the mesentery and peritoneal exudate, mirrored by blood neutrophilia. These changes subsided after 24 h. Ultrastructural immunocytochemical analysis of PMNs showed significant Gal-1 expression and co-localization with L-selectin and β2-integrin in the plasma membrane and cytoplasm. Pharmacological treatment with hrGal-1 at 4 h produced an inhibition of PMN migration, associated with diminished expression of adhesion molecules, particularly β2-integrin, and TNF-α and IL-1β release by peritoneal cells. At 24 h, Gal-1 induced an increase in mononuclear phagocytic cell recruitment. In conclusion, our data propose an important mechanism of anti-inflammatory action of Gal-1, initially by modulation of proinflammatory cytokine release and PMN migration through an imbalance between adhesion molecule expression and, later, by promoting monocyte-macrophage recruitment.-
Descrição: dc.descriptionDepartment of Anatomy São José do Rio Preto School of Medicine - FAMERP, São José do Rio Preto, SP-
Descrição: dc.descriptionDepartment of Morphology and Genetics Federal University of São Paulo - UNIFESP, São Paulo, SP-
Descrição: dc.descriptionDepartment of Biology Instituto de Biociências, Letras e Ciências Exatas São Paulo State University - UNESP, São José do Rio Preto, SP-
Descrição: dc.descriptionDepartment of Morphology and Genetics Federal University of São Paulo - UNIFESP, Rua Botucatu 740, 04023-900, São Paulo, SP-
Descrição: dc.descriptionDepartment of Biology Instituto de Biociências, Letras e Ciências Exatas São Paulo State University - UNESP, São José do Rio Preto, SP-
Formato: dc.format74-84-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Clinical and Experimental Pathology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCD11b-
Palavras-chave: dc.subjectCD62L-
Palavras-chave: dc.subjectImmunocytochemistry-
Palavras-chave: dc.subjectMonocyte-
Palavras-chave: dc.subjectNeutrophil-
Palavras-chave: dc.subjectZymosan peritonitis-
Título: dc.titleEffect of exogenous galectin-1 on leukocyte migration: Modulation of cytokine levels and adhesion molecules-
Tipo de arquivo: dc.typelivro digital-
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