Effects of light-curing time on the cytotoxicity of a restorative composite resin on odontoblast-like cells

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorVila Velha University Center-
Autor(es): dc.creatorAranha, Andreza Maria Fábio-
Autor(es): dc.creatorGiro, Elisa Maria Aparecida-
Autor(es): dc.creatorHebling, Josimeri-
Autor(es): dc.creatorLessa, Fernanda Campos Rosetti-
Autor(es): dc.creatorCosta, Carlos Alberto de Souza-
Data de aceite: dc.date.accessioned2025-08-21T21:31:35Z-
Data de disponibilização: dc.date.available2025-08-21T21:31:35Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2010-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/S1678-77572010000500006-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/226137-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/226137-
Descrição: dc.descriptionThis in vitro study evaluated the cytotoxicity of an experimental restorative composite resin subjected to different light-curing regimens. Methods: Forty round-shaped specimens were prepared and randomly assigned to four experimental groups (n=10), as follows: in Group 1, no light-curing; in Groups 2, 3 and 4, the composite resin specimens were light-cured for 20, 40 or 60 s, respectively. In Group 5, filter paper discs soaked in 5 μL PBS were used as negative controls. The resin specimens and paper discs were placed in wells of 24-well plates in which the odontoblast-like cells MDPC-23 (30,000 cells/cm2) were plated and incubated in a humidified incubator with 5% C02 and 95% air at 37°C for 72 h. The cytotoxicity was evaluated by the cell metabolism (MTT assay) and cell morphology (SEM). The data were analyzed statistically by Kruskal-Wallis and Mann-Whitney tests (p<0.05). Results: In G1, cell metabolism decreased by 86.2%, indicating a severe cytotoxicity of the non-light-cured composite resin. On the other hand, cell metabolism decreased by only 13.3% and 13.5% in G2 and G3, respectively. No cytotoxic effects were observed in G4 and G5. In G1, only a few round-shaped cells with short processes on their cytoplasmic membrane were observed. In the other experimental groups as well as in control group, a number of spindle-shaped cells with long cytoplasmic processes were found. Conclusion: Regardless of the photoactivation time used in the present investigation, the experimental composite resin presented mild to no toxic effects to the odontoblast-like MDPC-23 cells. However, intense cytotoxic effects occurred when no light-curing was performed.-
Descrição: dc.descriptionDepartment of Biology Science University of São Paulo Bauru School of Dentistry, Bauru, SP-
Descrição: dc.descriptionDiscipline of Pediatric Dentistry Department of Orthodontics and Pediatric Dentistry São Paulo State University Araraquara Dental School, Araraquara, SP-
Descrição: dc.descriptionDiscipline of Pathology Vila Velha University Center, Vitória, ES-
Descrição: dc.descriptionDiscipline of Pathology Department of Physiology and Pathology São Paulo State University Araraquara Dental School, Araraquara, SP-
Descrição: dc.descriptionDiscipline of Pediatric Dentistry Department of Orthodontics and Pediatric Dentistry São Paulo State University Araraquara Dental School, Araraquara, SP-
Descrição: dc.descriptionDiscipline of Pathology Department of Physiology and Pathology São Paulo State University Araraquara Dental School, Araraquara, SP-
Formato: dc.format461-466-
Idioma: dc.languageen-
Relação: dc.relationJournal of Applied Oral Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCultured cells-
Palavras-chave: dc.subjectCytotoxicity-
Palavras-chave: dc.subjectDental materials-
Palavras-chave: dc.subjectDental pulp-
Título: dc.titleEffects of light-curing time on the cytotoxicity of a restorative composite resin on odontoblast-like cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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