Toxicity of chlorhexidine on odontoblast-like cells

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorLessa, Fernanda Campos Rosetti-
Autor(es): dc.creatorAranha, Andreza Maria Fabio-
Autor(es): dc.creatorNogueira, Indri-
Autor(es): dc.creatorGiro, Elisa Maria Aparecida-
Autor(es): dc.creatorHebling, Josimeri-
Autor(es): dc.creatorCosta, Carlos Alberto de Souza-
Data de aceite: dc.date.accessioned2025-08-21T19:29:07Z-
Data de disponibilização: dc.date.available2025-08-21T19:29:07Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2010-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/S1678-77572010000100010-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/225829-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/225829-
Descrição: dc.descriptionChlorhexidine gluconate (CHX) is recommended for a number of clinical procedures and it has been pointed out as a potential cavity cleanser to be applied before adhesive restoration of dental cavities. Objective: As CHX may diffuse through the dentinal tubules to reach a monolayer of odontoblasts that underlies the dentin substrate, this study evaluated the cytotoxic effects of different concentrations of CHX on cultured odontoblast-like cells (MDPC- 23). Material and Methods: Cells were cultured and exposed to CHX solutions at concentrations of 0.06%, 0.12%, 0.2%, 1% and 2%. Pure culture medium (α-MEM) and 3% hydrogen peroxide were used as negative and positive control, respectively. After exposing the cultured cells to the controls and CHX solutions for 60 s, 2 h or 60 s with a 24- h recovery period, cell metabolism (MTT assay) and total protein concentration were evaluated. Cell morphology was assessed under scanning electron microscopy. CHX had a dose-dependent toxic effect on the MDPC-23 cells. Results: Statistically significant difference was observed when the cells were exposed to CHX in all periods (p<0.05). Significant difference was also determined for all CHX concentrations (p<0.05). The 60-s exposure time was the least cytotoxic (p<0.05), while exposure to CHX for 60 s with a 24-h recovery period was the most toxic to the cells (p<0.05). Conclusion: Regardless of the exposure time, all CHX concentrations had a high direct cytotoxic effect to cultured MDPC-23 cells.-
Descrição: dc.descriptionDiscipline of Pediatric Dentistry Department of Orthodontics and Pediatric Dentistry São Paulo State University Araraquara Dental School, Araraquara, SP-
Descrição: dc.descriptionDiscipline of Pathology Department of Physiology and Pathology São Paulo State University Araraquara Dental School, Araraquara, SP-
Descrição: dc.descriptionDiscipline of Pediatric Dentistry Department of Orthodontics and Pediatric Dentistry São Paulo State University Araraquara Dental School, Araraquara, SP-
Descrição: dc.descriptionDiscipline of Pathology Department of Physiology and Pathology São Paulo State University Araraquara Dental School, Araraquara, SP-
Formato: dc.format50-58-
Idioma: dc.languageen-
Relação: dc.relationJournal of Applied Oral Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCell viability-
Palavras-chave: dc.subjectChlorhexidine-
Palavras-chave: dc.subjectCytotoxicity-
Palavras-chave: dc.subjectOdontoblasts-
Palavras-chave: dc.subjectProtein synthesis-
Título: dc.titleToxicity of chlorhexidine on odontoblast-like cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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