Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorTakahagi-Nakaira, E.-
Autor(es): dc.creatorSugizaki, M. F.-
Autor(es): dc.creatorPeraçoli, Maria Terezinha S.-
Data de aceite: dc.date.accessioned2025-08-21T17:32:39Z-
Data de disponibilização: dc.date.available2025-08-21T17:32:39Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2009-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/S1678-91992009000400010-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/225737-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/225737-
Descrição: dc.descriptionIn vitro tests employing microdilution to evaluate fungal susceptibility to antifungal drugs are already standardized for fermentative yeasts. However, studies on the susceptibility of dimorphic fungi such as Paracoccidioides brasiliensis employing this method are scarce. The present work introduced some modifications into antifungal susceptibility testing from the European Committee on Antimicrobial Susceptibility Testing (EUCAST), concerning broth medium and reading time, to determine minimal inhibitory concentration (MIC) of amphotericin B and itraconazole against Paracoccidioides brasiliensis. Yeast-like cells of P. brasiliensis (Pb18 strain) were tested for susceptibility to amphotericin B and itraconazole in RPMI 1640 medium, supplemented with 2% glucose and nitrogen source and incubated at 35°C. The MIC of amphotericin B and itraconazole against Pb18 were respectively 0.25 μg/mL and 0.002 μg/mL. The results of minimal fungicidal concentration (MFC) showed that amphotericin B at 0.25 μg/mL or higher concentrations displayed fungicidal activity against Pb18 while itraconazole at least 0.002 μg/mL has a fungistatic effect on P. brasiliensis. In conclusion, our results showed that the method employed in the present study is reproducible and reliable for testing the susceptibility of P. brasiliensis to antifungal drugs.-
Descrição: dc.descriptionDepartamento de Microbiologia e Imunologia Instituto de Biociências UNESP, Botucatu, SP, 18618-970-
Descrição: dc.descriptionDepartamento de Microbiologia e Imunologia Instituto de Biociências UNESP, Botucatu, SP, 18618-970-
Formato: dc.format718-731-
Idioma: dc.languageen-
Relação: dc.relationJournal of Venomous Animals and Toxins Including Tropical Diseases-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAmphotericin B-
Palavras-chave: dc.subjectAntifungal susceptibility-
Palavras-chave: dc.subjectItraconazole-
Palavras-chave: dc.subjectP. brasiliensis-
Título: dc.titleMicrodilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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