Characterization and polymorphism screening of IGF-I and prolactin genes in Nelore heifers

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.contributorCentro Nacional de Desenvolvimento Científico e Tecnológico-
Autor(es): dc.contributorInstituto de Ciência e Tecnologia - Ciência Animal-
Autor(es): dc.creatorLaureano, Monyka Marianna Massolini-
Autor(es): dc.creatorOtaviano, Antônio Roberto-
Autor(es): dc.creatorLima, André Luís Ferreira-
Autor(es): dc.creatorCosta, Raphael Bermal-
Autor(es): dc.creatorSalman, Ana Karina Dias-
Autor(es): dc.creatorSena, Janete Apparecida Desidério-
Autor(es): dc.creatorTonhati, Humberto-
Autor(es): dc.creatorde Albuquerque, Lúcia Galvão-
Data de aceite: dc.date.accessioned2025-08-21T21:04:19Z-
Data de disponibilização: dc.date.available2025-08-21T21:04:19Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2009-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.4081/ijas.2009.277-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/225578-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/225578-
Descrição: dc.descriptionInsulin growth factor I (IGF-I) and prolactin (PRL) are peptide hormones that exert complementary effects on reproductive traits by acting on folliculogenesis. In view of the lack of information about the IGF-I and PRL genes in Bos indicus, the objective of this study was to partially characterize the promoter regions of these genes and to screen animals of different ages at first pregnancy for the presence of polymorphisms in these regions. In addition, we determined whether polymorphisms influence the regulation of the two hormone genes, evaluating their association with sexual precocity. The animals were divided into three groups according to age at first pregnancy: 1) 100 heifers considered to be sexually precocious that became pregnant at 15-16 months of age, 2) 100 heifers that became pregnant during the normal breeding season at 24 months of age, and 3) 100 heifers that did not become pregnant until 24 months of age. For the IGF-I gene, PCR-RFLP-SnaBI analysis showed the presence of genotypes AB and BB at frequencies of 0.02 and 0.98, respectively. Sequencing of the IGF-I gene fragment revealed a single nitrogen base change from cytosine to thymine, corresponding to the restriction site of SnaBI. The polymorphisms identified in the 5'-flanking region of the IGF-I gene may serve as a basis for future studies of molecular markers in cattle. For the PRL gene, PCR-RFLP-HaeIII analysis showed the presence of only one migration pattern, a finding characterizing the region studied as monomorphic. The study of other regions in the IGF-I and PRL genes might provide molecular data that can be used in the future for the selection of sexually precocious animals.-
Descrição: dc.descriptionDepartamento de Zootecnia Universidade Estadual Paulista, Jaboticabal-
Descrição: dc.descriptionCentro de Pesquisa Agroflorestais-Embrapa Rondônia, Porto Velho-
Descrição: dc.descriptionDepartamento de Biologia Aplicada à Agropecuária Universidade Estadual Paulista, Jaboticabal-
Descrição: dc.descriptionCentro Nacional de Desenvolvimento Científico e Tecnológico, Brasilia-
Descrição: dc.descriptionInstituto de Ciência e Tecnologia - Ciência Animal-
Descrição: dc.descriptionDepartamento de Zootecnia Universidade Estadual Paulista, Jaboticabal-
Descrição: dc.descriptionDepartamento de Biologia Aplicada à Agropecuária Universidade Estadual Paulista, Jaboticabal-
Formato: dc.format277-283-
Idioma: dc.languageit-
Relação: dc.relationItalian Journal of Animal Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBeef cattle-
Palavras-chave: dc.subjectMolecular marker-
Palavras-chave: dc.subjectRFLP-
Palavras-chave: dc.subjectSexual precocity-
Título: dc.titleCharacterization and polymorphism screening of IGF-I and prolactin genes in Nelore heifers-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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