Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART)

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorMeira, D. A.-
Autor(es): dc.creatorRamb, Almeida-
Autor(es): dc.creatorBarbosa, A. N.-
Autor(es): dc.creatorDe Souza, L. R.-
Autor(es): dc.creatorTet, Olivo-
Autor(es): dc.creatorHenriques, R. M.S.-
Autor(es): dc.creatorGolim, M. A.-
Autor(es): dc.creatorAraújo, J. R.J.P.-
Autor(es): dc.creatorNagoshi, L. R.-
Autor(es): dc.creatorOrikaza, C. M.-
Autor(es): dc.creatorCalvi, S. A.-
Data de aceite: dc.date.accessioned2025-08-21T22:49:24Z-
Data de disponibilização: dc.date.available2025-08-21T22:49:24Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2008-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/S1678-91992008000400011-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/225378-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/225378-
Descrição: dc.descriptionA cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G1 = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G2 = 27); and patients on HAART with undetectable VL for at least the past six months (G3 = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G4 = 20), which was the control group. Serum cytokine levels (values in pg/mL) were measured by enzyme-linked immunosorbent assay (ELISA) and specific mRNA expression by reverse transcription polymerase chain reaction (RT-PCR). Both techniques were performed on the four groups for TNF-α, IL-2, INF-γ, IL-4 and IL-10. All patients were submitted to VL determination and CD4+ and CD8+T lymphocyte counts. The analysis of the results revealed a significant comparison among groups for both methods and an association between the latter (> 80% -r2 > 0.80). There was only one exception, in control individuals for IL-2 by ELISA. The cytokine profiles, in both methods, for the three patient groups, were mature Th-0. The behaviors of IL-2 and INF-γ required emphasis due to consequent expression of dominant Th profile. Both methods showed low IL-2 and high mean values of INF-γ in the three groups. Several authors have recently drawn attention to the substantial apoptosis of infected and non-infected CD4+T cells, mainly during primary infection, persisting only in those with INF-γ phenotype producer and not IL-2. HIV infected individuals submitted to HAART are expected to produce IL-2 in an attempt to present Th-1 profile, but in most cases this did not occur.-
Descrição: dc.descriptionDepartment of Tropical Diseases São Paulo State University UNESP, Botucatu, São Paulo State-
Descrição: dc.descriptionDepartment of Microbiology and Immunology Sao Paulo State University UNESP, Botucatu, Sao Paulo State-
Descrição: dc.descriptionDepartamento de Doenças Tropicais e Diagnóstics por Imagem Faculdade de Medicina de Botucatu UNESP, Distrito de Rubião Júnior s/n, Botucatu, SP, 18618-000, Brasil-
Descrição: dc.descriptionDepartment of Tropical Diseases São Paulo State University UNESP, Botucatu, São Paulo State-
Descrição: dc.descriptionDepartment of Microbiology and Immunology Sao Paulo State University UNESP, Botucatu, Sao Paulo State-
Descrição: dc.descriptionDepartamento de Doenças Tropicais e Diagnóstics por Imagem Faculdade de Medicina de Botucatu UNESP, Distrito de Rubião Júnior s/n, Botucatu, SP, 18618-000, Brasil-
Formato: dc.format685-702-
Idioma: dc.languageen-
Relação: dc.relationJournal of Venomous Animals and Toxins Including Tropical Diseases-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAIDS-
Palavras-chave: dc.subjectApoptosis-
Palavras-chave: dc.subjectCytokines-
Palavras-chave: dc.subjectELISA-
Palavras-chave: dc.subjectHAART-
Palavras-chave: dc.subjectHIV-
Palavras-chave: dc.subjectRT-PCR-
Título: dc.titleAssessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART)-
Tipo de arquivo: dc.typelivro digital-
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