Role of Paracoccidioides brasiliensis cell wall fraction containing β-glucan in tumor necrosis factor-alpha production by human monocytes: Correlation with fungicidal activity

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorAnjos, A. R.-
Autor(es): dc.creatorCalvi, S. A.-
Autor(es): dc.creatorFerracini, R.-
Autor(es): dc.creatorPeraçoli, M. T.S.-
Autor(es): dc.creatorSilva, C. L.-
Autor(es): dc.creatorSoares, A. M.V.C.-
Data de aceite: dc.date.accessioned2025-08-21T19:51:57Z-
Data de disponibilização: dc.date.available2025-08-21T19:51:57Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2002-01-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/mmy.40.4.377.382-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/224277-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/224277-
Descrição: dc.descriptionThe polysaccharide fraction of Paracoccidioides brasiliensis mycelial cell wall (F1 fraction), the active component of which is composed of β-glucan, was investigated in regard to the activation of human monocytes for fungal killing. The cells were primed with interferon-γ (IFN-γ) or F1 (100 and 200 μg ml-1) or F1 (100 and 200 μg ml-1) plus IFN-γ for 24 h and then evaluated for H2O2release. In other experiments, the cells were pretreated with the same stimuli, challenged with a virulent strain of P. brasiliensis and evaluated for fungicidal activity and levels of tumor necrosis factor (TNF-α) in the supernatants. F1 increased the levels of H2O2in a similar manner to IFN-γ. However, a synergistic effect between these two activators was not detected. On the contrary, a significant fungicidal activity was only obtained after priming with IFN-γ plus F1. This higher activity was associated with high levels of TNF-α in the supernatants of the cocultures. Overall, P. brasiliensis F1 fraction induced human monocytes to release relatively high levels of TNF-α, which, in combination with IFN-γ, is responsible for the activation of human monocytes for effective killing of P. brasiliensis.-
Descrição: dc.descriptionDepartment of Microbiology and Immunology Biosciences Institute São Paulo State University-Botucatu, SP-
Descrição: dc.descriptionDepartment of Parasitology Microbiology and Immunology School of Medicine of Ribeirão Preto University of São Paulo, SP-
Descrição: dc.descriptionDepartment of Microbiology and Immunology Biosciences Institute São Paulo State University-Botucatu, SP-
Formato: dc.format377-382-
Idioma: dc.languageen-
Relação: dc.relationMedical Mycology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectFungicidal activity-
Palavras-chave: dc.subjectMonocyte-
Palavras-chave: dc.subjectParacoccidioides brasiliensis-
Palavras-chave: dc.subjectTNF-
Palavras-chave: dc.subjectβ-Glucan-
Título: dc.titleRole of Paracoccidioides brasiliensis cell wall fraction containing β-glucan in tumor necrosis factor-alpha production by human monocytes: Correlation with fungicidal activity-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.