Advantages of using nested collision induced dissociation/post-source decay with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: Sequencing of novel peptides from wasp venom

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Autor(es): dc.contributorSuntory Institute for Bioorganic Research-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorColumbia University-
Autor(es): dc.creatorHisada, Miki-
Autor(es): dc.creatorKonno, Katsuhiro-
Autor(es): dc.creatorItagaki, Yasuhiro-
Autor(es): dc.creatorNaoki, Hideo-
Autor(es): dc.creatorNakajima, Terumi-
Data de aceite: dc.date.accessioned2025-08-21T20:37:34Z-
Data de disponibilização: dc.date.available2025-08-21T20:37:34Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2000-10-13-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/1097-0231(20001015)14:19<1828-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/224154-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/224154-
Descrição: dc.descriptionWe have examined the applicability of the 'nested' collision induced dissociation/post-source decay (CID/PSD) method to the sequencing of novel peptides from solitary wasps which have neurotoxic venom for paralyzing other insects. The CID/PSD spectrum of a ladder peptide derived from an exopeptidase digest was compared with that of the intact peptide. The mass peaks observed only in the CID/PSD spectrum of a ladder peptide were extracted as C-terminal fragment ions. Assignment of C-terminal fragment ions enabled calculation of N-terminal fragment masses, leading to differentiation between N-terminal fragment ions and internal fragment ions. This methodology allowed rapid and sensitive identification by removing ambiguity in the assignment of the fragment ions, and proved useful for sequencing unknown peptides, in particular those available as natural products with a limited supply. Copyright (C) 2000 John Wiley and Sons, Ltd.-
Descrição: dc.descriptionSuntory Institute for Bioorganic Research, Wakayamadai 1-1-1, Shimamoto-cho, Mishima-gun, Osaka 618-8503-
Descrição: dc.descriptionLaboratory of Structural Biology and Zoochemistry Institute of Biosciences of Rio Claro Sao Paulo State University (UNESP), Av. 24-A, 1515 Bela Vista, Rio Claro, SP 13506-900-
Descrição: dc.descriptionDepartment of Chemistry MC3183 Columbia University, New York, NY 10027-
Descrição: dc.descriptionLaboratory of Structural Biology and Zoochemistry Institute of Biosciences of Rio Claro Sao Paulo State University (UNESP), Av. 24-A, 1515 Bela Vista, Rio Claro, SP 13506-900-
Formato: dc.format1828-1834-
Idioma: dc.languageen-
Relação: dc.relationRapid Communications in Mass Spectrometry-
???dc.source???: dc.sourceScopus-
Título: dc.titleAdvantages of using nested collision induced dissociation/post-source decay with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: Sequencing of novel peptides from wasp venom-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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