Localization of 5-methylcytosine in metaphase chromosomes of diploid and triploid pacu fish, Piaractus mesopotamicus (Pisces, Characiformes)

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorHôpital Necker-Enfants Malades-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorCNRS-
Autor(es): dc.creatorDe Almeida-Toledo, L. F.-
Autor(es): dc.creatorViegas-Péquignot, E.-
Autor(es): dc.creatorCoutinho-Barbosa, A. C.-
Autor(es): dc.creatorForesti, F.-
Autor(es): dc.creatorNiveleau, A.-
Autor(es): dc.creatorDe Almeida Toledo-Filho, S.-
Data de aceite: dc.date.accessioned2025-08-21T15:16:29Z-
Data de disponibilização: dc.date.available2025-08-21T15:16:29Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued1998-12-01-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/224110-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/224110-
Descrição: dc.descriptionThe distribution of 5-methylcytosine (5-MeC) was investigated in fish chromosomes by indirect immunofluorescence using a highly specific 5-MeC monoclonal antibody. Diploid and artificially produced triploid specimens of the pacu fish, Piaractus mesopotamicus, were analyzed. The strong immunofluorescent signals were coincident with the heterochromatic regions of both diploids and triploids in a pattern that matched the C-banding pattern. In the euchromatin, heterogeneous labeling was observed along the chromatids. The weakness of this labeling hindered comparison of the fluorescence labeling of homologous chromosomes from diploid and triploid individuals. However, no striking differences were observed. The possibility that the euchromatin labeling by the 5-MeC antibody is related to the occurrence of mildly repetitive sequences in the genome of Piaractus is discussed.-
Descrição: dc.descriptionDepartamento de Biologia Instituto de Biociências Universidade de São Paulo, São Paulo-
Descrição: dc.descriptionU 383 INSERM Hôpital Necker-Enfants Malades, Paris-
Descrição: dc.descriptionDepartamento de Morfologia Instituto de Biociências UNESP, Botucatu-
Descrição: dc.descriptionInstitut Pasteur CNRS, Lyon-
Descrição: dc.descriptionDepartamento de Biologia Instituto de Biociências USP, Calxa Postal 11.461, 05422-970 São Paulo, SP-
Descrição: dc.descriptionDepartamento de Morfologia Instituto de Biociências UNESP, Botucatu-
Formato: dc.format21-24-
Idioma: dc.languageen-
Relação: dc.relationCytogenetics and Cell Genetics-
???dc.source???: dc.sourceScopus-
Título: dc.titleLocalization of 5-methylcytosine in metaphase chromosomes of diploid and triploid pacu fish, Piaractus mesopotamicus (Pisces, Characiformes)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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