How adaptive laboratory evolution can boost yeast tolerance to lignocellulosic hydrolyses

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorMenegon, Yasmine Alves-
Autor(es): dc.creatorGross, Jeferson-
Autor(es): dc.creatorJacobus, Ana Paula-
Data de aceite: dc.date.accessioned2025-08-21T16:32:23Z-
Data de disponibilização: dc.date.available2025-08-21T16:32:23Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00294-022-01237-z-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/223749-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/223749-
Descrição: dc.descriptionThe yeast Saccharomyces cerevisiae is an excellent candidate for establishing cell factories to convert lignocellulosic biomass into chemicals and fuels. To enable this technology, yeast robustness must be improved to withstand the fermentation inhibitors (e.g., weak organic acids, phenols, and furan aldehydes) resulting from biomass pretreatment and hydrolysis. Here, we discuss how evolution experiments performed in the lab, a method commonly known as adaptive laboratory evolution (ALE), may contribute to lifting yeast tolerance against the inhibitors of lignocellulosic hydrolysates (LCHs). The key is that, through the combination of whole-genome sequencing and reverse engineering, ALE provides a robust platform for discovering and testing adaptive alleles, allowing to explore the genetic underpinnings of yeast responses to LCHs. We review the insights gained from past evolution experiments with S. cerevisiae in LCH inhibitors and propose experimental designs to optimise the discovery of genetic variants adaptive to biomass toxicity. The knowledge gathered through ALE projects is envisaged as a roadmap to engineer superior yeast strains for biomass-based bioprocesses.-
Descrição: dc.descriptionInstitute for Bioenergy Research São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionInstitute for Bioenergy Research São Paulo State University (UNESP), São Paulo-
Idioma: dc.languageen-
Relação: dc.relationCurrent Genetics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAdaptive laboratory evolution-
Palavras-chave: dc.subjectLignocellulosic hydrolysates-
Palavras-chave: dc.subjectMolecular genetics-
Palavras-chave: dc.subjectSaccharomyces cerevisiae-
Palavras-chave: dc.subjectSecond-generation ethanol-
Título: dc.titleHow adaptive laboratory evolution can boost yeast tolerance to lignocellulosic hydrolyses-
Tipo de arquivo: dc.typevídeo-
Aparece nas coleções:Repositório Institucional - Unesp

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