Inflammasome NLRP3 activation induced by Convulxin, a C-type lectin-like isolated from Crotalus durissus terrificus snake venom

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorFIOCRUZ Rondônia-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFederal University of Amapá-
Autor(es): dc.contributorUNIR-
Autor(es): dc.creatorRego, Cristina M. A.-
Autor(es): dc.creatorFrancisco, Aleff F.-
Autor(es): dc.creatorBoeno, Charles N.-
Autor(es): dc.creatorPaloschi, Mauro V.-
Autor(es): dc.creatorLopes, Jéssica A.-
Autor(es): dc.creatorSilva, Milena D. S.-
Autor(es): dc.creatorSantana, Hallison M.-
Autor(es): dc.creatorSerrath, Suzanne N.-
Autor(es): dc.creatorRodrigues, Jaína E.-
Autor(es): dc.creatorLemos, Caleb T. L.-
Autor(es): dc.creatorDutra, Ricardo S. S.-
Autor(es): dc.creatorda Cruz, Jorddy N.-
Autor(es): dc.creatordos Santos, Cleydson Breno R.-
Autor(es): dc.creatorda S. Setúbal, Sulamita-
Autor(es): dc.creatorFontes, Marcos R. M.-
Autor(es): dc.creatorSoares, Andreimar M.-
Autor(es): dc.creatorPires, Weverson L.-
Autor(es): dc.creatorZuliani, Juliana P.-
Data de aceite: dc.date.accessioned2025-08-21T15:43:04Z-
Data de disponibilização: dc.date.available2025-08-21T15:43:04Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-022-08735-7-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/223680-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/223680-
Descrição: dc.descriptionConvulxin (CVX), a C-type lectin-like protein isolated from the venom of the snake species, Crotalus durissus terrificus, stimulates platelet aggregation by acting as a collagen receptor agonist for glycoprotein VI found in the platelets. The effect of CVX on platelets has been studied, but its effect on human peripheral blood mononuclear cells (PBMCs) remains unclear. Given the significance of PBMCs in inflammation, this study explored the effect of CVX on PBMCs, specifically regarding NLRP3 inflammasome activation by assessing cell viability, ability to induce cell proliferation, reactive oxygen species (ROS) and nitric oxide production, interleukin (IL)-2 and IL-10 secretion, NLRP3 complex activation, and the role of C-type lectin-like receptors (CTLRs) in these. CVX was not toxic to PBMCs at the investigated concentrations and did not increase PBMC growth or IL-2 release; however, CVX induced IL-10 release and ROS generation via monocyte activation. It also activated the NLRP3 complex, resulting in IL-1β induction. Furthermore, the interaction between CVX and Dectin-2, a CTLR, induced IL-10 production. CVX interaction with CTLR has been demonstrated by laminarin therapy. Because of the involvement of residues near the Dectin-2 carbohydrate-recognition site, the generation of ROS resulted in inflammasome activation and IL-1β secretion. Overall, this work helps elucidate the function of CVX in immune system cells.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionLaboratory of Cellular Immunology Applied to Health Oswaldo Cruz Foundation FIOCRUZ Rondônia, RO-
Descrição: dc.descriptionDepartment of Physics and Biophysics Institute of Biosciences São Paulo State University UNESP, SP-
Descrição: dc.descriptionLaboratory of Modeling and Computational Chemistry Department of Biological and Health Sciences Federal University of Amapá, AP-
Descrição: dc.descriptionCenter of Biomolecules Applied to Health (CEBio) Fundação Oswaldo Cruz FIOCRUZ Rondônia, RO-
Descrição: dc.descriptionDepartment of Medicine Federal University of Rondônia UNIR, RO-
Descrição: dc.descriptionDepartment of Physics and Biophysics Institute of Biosciences São Paulo State University UNESP, SP-
Descrição: dc.descriptionCNPq: 306197/2017-0-
Descrição: dc.descriptionCNPq: 306672/2014-6-
Descrição: dc.descriptionCNPq: 479316-2013-6-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Título: dc.titleInflammasome NLRP3 activation induced by Convulxin, a C-type lectin-like isolated from Crotalus durissus terrificus snake venom-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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