Serine proteases in neutrophil extracellular traps exhibit anti-Respiratory Syncytial Virus activity

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.contributorUniversity of Manitoba-
Autor(es): dc.contributorFederal University of Jatai-
Autor(es): dc.contributorFederal University of Juiz de Fora-
Autor(es): dc.creatorLopes, Bruno Rafael Pereira-
Autor(es): dc.creatorda Silva, Gabriel Soares-
Autor(es): dc.creatorde Lima Menezes, Gabriela-
Autor(es): dc.creatorde Oliveira, Juliana-
Autor(es): dc.creatorWatanabe, Aripuanã Sakurada Aranha-
Autor(es): dc.creatorPorto, Bárbara Nery-
Autor(es): dc.creatorda Silva, Roosevelt Alves-
Autor(es): dc.creatorToledo, Karina Alves-
Data de aceite: dc.date.accessioned2025-08-21T20:43:43Z-
Data de disponibilização: dc.date.available2025-08-21T20:43:43Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.intimp.2022.108573-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/223465-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/223465-
Descrição: dc.descriptionHuman respiratory syncytial virus (hRSV) is an infectious agent in infants and young children which there are no vaccines or drugs for treatment. Neutrophils are recruited for airway, where they are stimulated by hRSV to release large amounts of neutrophil extracellular traps (NETs). NETs are compound by DNA and proteins, including microbicidal enzymes. They constitute a large part of the mucus accumulated in the lung of patients, compromising their breathing capacity. In contrast, NETs can capture/inactivate hRSV, but the molecules responsible for this effect are unknown. Objectives: We selected microbicidal NET enzymes (elastase, myeloperoxidase, cathepsin-G, and proteinase-3) to assess their anti-hRSV role. Methods and Results: Through in vitro assays using HEp-2 cells, we observed that elastase, proteinase-3, and cathepsin-G, but not myeloperoxidase, showed virucidal effects even at non-cytotoxic concentrations. Elastase and proteinase-3, but not cathepsin-G, cleaved viral F-protein, which is responsible for viral adhesion and fusion with the target cells. Molecular docking analysis indicated the interaction of these macromolecules in the antigenic regions of F-protein through the active regions of the enzymes. Conclusions: Serine proteases from NETs interact and inactive hRSV. These results contribute to the understanding the role of NETs in hRSV infection and to designing treatment strategies for the inflammatory process during respiratory infections.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Sciences-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Sciences Humanities and Languages-
Descrição: dc.descriptionGraduate Program in Applied and Computational Mathematics – PGMAC - State University of Londrina-
Descrição: dc.descriptionDepartment of Medical Microbiology and Infectious Diseases University of Manitoba-
Descrição: dc.descriptionBiosystems Collaborative Nucleus Institute of Exact Sciences Federal University of Jatai-
Descrição: dc.descriptionVirology Laboratory Center for Microbiology Studies Department of Parasitology Microbiology and Immunology Federal University of Juiz de Fora, Minas Gerais-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Sciences-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Sciences Humanities and Languages-
Descrição: dc.descriptionFAPESP: 2018/09021-4-
Idioma: dc.languageen-
Relação: dc.relationInternational Immunopharmacology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectNeutrophils-
Palavras-chave: dc.subjectProteases-
Palavras-chave: dc.subjectRespiratory syncytial virus-
Palavras-chave: dc.subjectVirucidal-
Título: dc.titleSerine proteases in neutrophil extracellular traps exhibit anti-Respiratory Syncytial Virus activity-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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