Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorRMIT University-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorAustralian Synchrotron-
Autor(es): dc.contributorCIEPQPF-
Autor(es): dc.creatorVeríssimo, Nathalia V.-
Autor(es): dc.creatorSaponi, Carolina F.-
Autor(es): dc.creatorRyan, Timothy M.-
Autor(es): dc.creatorGreaves, Tamar L.-
Autor(es): dc.creatorPereira, Jorge F.B.-
Data de aceite: dc.date.accessioned2025-08-21T22:06:17Z-
Data de disponibilização: dc.date.available2025-08-21T22:06:17Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2021-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.gce.2021.08.001-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/223399-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/223399-
Descrição: dc.descriptionFluorescent Proteins (FP) can be applied as biomarkers and biosensors in the industrial and medical fields, but their large-scale use, especially for new industrial applications, is limited due to their low stability. Hence, the discovery of additives capable of preserving the activity of FP at room temperature and under stress conditions can help to expand and facilitate their commercial use. With this goal, we evaluated the application of 1-alkyl-3-methylimidazolium chloride-based ([Cnmim]Cl) ionic liquids (ILs) as additives to preserve the activity of Enhanced Green Fluorescent Protein (EGFP) at different storage times and under unfavorable conditions. All [Cnmim]Cl solutions (at 0.100 mol L−1) were able to preserve EGFP fluorescence for longer than the phosphate-saline buffer (PBS) and NaCl solutions, increasing its fluorescence manifestation from 1 to 3 months. [Cnmim]Cl with shorter to medium cationic alkyl chains were the most effective in preserving EGFP fluorescence. [Cnmim]Cl also protected EGFP activity in the presence of the surfactant SDS, the acid guanidine hydrochloride, and H2O2. Therefore, [Cnmim]Cl can be added to aqueous solutions to preserve EGFP fluorescence activity at room temperature for longer storage times and to reduce the negative impact of denaturing agents on EGFP. Therefore, there is a massive potential for the application of ILs as additives to preserve FP in the long-term without refrigeration and under unfavorable conditions, and this is fundamental to enable expansion of FP in industrial and commercial applications.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionASCRS Research Foundation-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia-
Descrição: dc.descriptionDepartment of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP), Rodovia Araraquara-Jaú/Km 01-
Descrição: dc.descriptionSchool of Science College of Science Engineering and Health RMIT University, 124 La Trobe Street-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences São Paulo University (USP), Av. Prof. Lineu Prestes, no. 580, B16, 05508-000, Cidade de Universitária-
Descrição: dc.descriptionAustralian Synchrotron, 800 Blackburn Road-
Descrição: dc.descriptionUniv Coimbra CIEPQPF Department of Chemical Engineering, Rua Sílvio Lima, Pólo II – Pinhal de Marrocos-
Descrição: dc.descriptionDepartment of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP), Rodovia Araraquara-Jaú/Km 01-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2014/16424–7-
Descrição: dc.descriptionFAPESP: 2014/19793–3-
Descrição: dc.descriptionFAPESP: 2016/07529–5-
Descrição: dc.descriptionFAPESP: 2018/01858–2-
Descrição: dc.descriptionFAPESP: 2018/06576–5-
Descrição: dc.descriptionFAPESP: 2018/20833–0-
Descrição: dc.descriptionFAPESP: 2018/25511–1-
Descrição: dc.descriptionFAPESP: 2018/50009–8-
Descrição: dc.descriptionFAPESP: 2020/14144–8-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia: UIDB/EQU/00102/2020-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia: UIDP/EQU/00102/2020-
Formato: dc.format412-422-
Idioma: dc.languageen-
Relação: dc.relationGreen Chemical Engineering-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectFluorescence-
Palavras-chave: dc.subjectGreen Fluorescent Protein-
Palavras-chave: dc.subjectIonic liquids-
Palavras-chave: dc.subjectPreservatives-
Palavras-chave: dc.subjectProtein stability-
Título: dc.titleImidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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