Expression of bta-miR-222 and LHCGR in bovine cultured granulosa cells: Impact of follicle deviation and regulation by FSH/insulin in vitro

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Western São Paulo (Unoeste)-
Autor(es): dc.creatorSantos, P. H.-
Autor(es): dc.creatorNunes, S. G.-
Autor(es): dc.creatorFranchi, F. F.-
Autor(es): dc.creatorGiroto, A. B.-
Autor(es): dc.creatorFontes, P. K.-
Autor(es): dc.creatorPinheiro, V. G.-
Autor(es): dc.creatorCastilho, A. C.S.-
Data de aceite: dc.date.accessioned2025-08-21T20:59:19Z-
Data de disponibilização: dc.date.available2025-08-21T20:59:19Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2022.01.034-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/223396-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/223396-
Descrição: dc.descriptionFinal antral follicle development and future ovulation are mediated by gonadotropin-induced changes with spatio-temporally regulated expression of genes. Here, we aimed to quantify the relative mRNA abundance of bta-miR-222 and its predicted target, LHCGR, in granulosa cells (GCs) from follicles, after follicle deviation, as well as from GCs cultured in vitro with follicle stimulating hormone (FSH) and/or insulin. Thus, to study the impact of follicle deviation, Nelore heifers (n = 10; Bos taurus indicus) were hormonally synchronized and slaughtered 3 days after ovulation. Then, GCs from the dominant follicle (DF) and its respective subordinate follicle (SF) were recovered for RT-qPCR. For in vitro analysis, small follicles (2–5 mm) were dissected from bovine ovaries collected from a local abattoir. The GCs were isolated and cultured in serum-free medium, or treated with insulin (1 ng/mL or 10 ng/mL) alone or in combination with human recombinant FSH (1 ng/mL), for 6 days. Our findings showed that the relative mRNA abundance of LHCGR in GCs was higher in the DF compared to the SF (p = 0.01). Inversely, bta-miR-222 expression was lower in the DF compared to the SF (p = 0.01). Furthermore, GCs cultured with FSH and insulin together resulted in a higher abundance of LHCGR and a lower abundance of bta-miR-222 (p ≤ 0.05) when compared to GCs cultured with insulin alone. In conclusion, we found that the LHCGR upregulation in GCs from the DF is inversely related to bta-miR-222 expression. We also suggest the involvement of FSH in bta-miR-222 suppression in healthy bovine GCs.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (Unesp) Department of Pharmacology Institute of Biosciences-
Descrição: dc.descriptionUniversity of Western São Paulo (Unoeste)-
Descrição: dc.descriptionSão Paulo State University (Unesp) Department of Pharmacology Institute of Biosciences-
Descrição: dc.descriptionFAPESP: 001-
Descrição: dc.descriptionFAPESP: 2013/11480-3-
Descrição: dc.descriptionFAPESP: 2015/04505-5-
Formato: dc.format71-77-
Idioma: dc.languageen-
Relação: dc.relationTheriogenology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntral follicle-
Palavras-chave: dc.subjectCattle-
Palavras-chave: dc.subjectFollicle divergence-
Palavras-chave: dc.subjectGene expression-
Palavras-chave: dc.subjectLHCGR-
Palavras-chave: dc.subjectMicroRNA-
Título: dc.titleExpression of bta-miR-222 and LHCGR in bovine cultured granulosa cells: Impact of follicle deviation and regulation by FSH/insulin in vitro-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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