Effects of Formyl Peptide Receptor Agonists Ac9-12 and WKYMV in In Vivo and In Vitro Acute Inflammatory Experimental Models

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Autor(es): dc.contributorUniversidade Federal de São Paulo (UNIFESP)-
Autor(es): dc.contributorUT Southwestern Medical Center-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorSao Francisco University-
Autor(es): dc.creatorLice, Izabella-
Autor(es): dc.creatorSanches, José Marcos-
Autor(es): dc.creatorCorreia-Silva, Rebeca D.-
Autor(es): dc.creatorCorrêa, Mab P.-
Autor(es): dc.creatorIcimoto, Marcelo Y.-
Autor(es): dc.creatorSilva, Alex A. R.-
Autor(es): dc.creatorSánchez-Vinces, Salvador-
Autor(es): dc.creatorPorcari, Andreia M.-
Autor(es): dc.creatorMoreira, Vanessa-
Autor(es): dc.creatorGil, Cristiane D.-
Data de aceite: dc.date.accessioned2025-08-21T18:04:59Z-
Data de disponibilização: dc.date.available2025-08-21T18:04:59Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/cells11020228-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/223235-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/223235-
Descrição: dc.descriptionFormyl peptide receptors (Fprs) are a G-protein-coupled receptor family mainly expressed on leukocytes. The activation of Fpr1 and Fpr2 triggers a cascade of signaling events, leading to leukocyte migration, cytokine release, and increased phagocytosis. In this study, we evaluate the effects of the Fpr1 and Fpr2 agonists Ac9-12 and WKYMV, respectively, in carrageenan-induced acute peritonitis and LPS-stimulated macrophages. Peritonitis was induced in male C57BL/6 mice through the intraperitoneal injection of 1 mL of 3% carrageenan solution or saline (control). Pre-treatments with Ac9-12 and WKYMV reduced leukocyte influx to the peritoneal cavity, particularly neutrophils and monocytes, and the release of IL-1β. The addition of the Fpr2 antagonist WRW4 reversed only the anti-inflammatory actions of WKYMV. In vitro, the administration of Boc2 and WRW4 reversed the effects of Ac9-12 and WKYMV, respectively, in the production of IL-6 by LPS-stimulated macrophages. These biological effects of peptides were differently regulated by ERK and p38 signaling pathways. Lipidomic analysis evidenced that Ac9-12 and WKYMV altered the intracellular lipid profile of LPS-stimulated macrophages, revealing an increased concentration of several glycerophospholipids, suggesting regulation of inflammatory pathways triggered by LPS. Overall, our data indicate the therapeutic potential of Ac9-12 and WKYMV via Fpr1 or Fpr2-activation in the inflammatory response and macrophage activation.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Morphology and Genetics Universidade Federal de São Paulo-UNIFESP, SP-
Descrição: dc.descriptionUT Southwestern Medical Center Department of Ophthalmology-
Descrição: dc.descriptionInstitute of Biosciences Humanities and Exact Sciences Universidade Estadual Paulista-UNESP, SP-
Descrição: dc.descriptionDepartment of Biophysics Universidade Federal de São Paulo-UNIFESP, SP-
Descrição: dc.descriptionMS4Life Laboratory of Mass Spectrometry Health Sciences Postgraduate Program Sao Francisco University, SP-
Descrição: dc.descriptionDepartment of Pharmacology Universidade Federal de São Paulo-UNIFESP, SP-
Descrição: dc.descriptionInstitute of Biosciences Humanities and Exact Sciences Universidade Estadual Paulista-UNESP, SP-
Descrição: dc.descriptionFAPESP: 2019/04314-6-
Descrição: dc.descriptionFAPESP: 2019/15017-2-
Descrição: dc.descriptionFAPESP: 2020/03565-2-
Idioma: dc.languageen-
Relação: dc.relationCells-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAnnexin A1-derived peptides-
Palavras-chave: dc.subjectCarrageenan-
Palavras-chave: dc.subjectLPS-
Palavras-chave: dc.subjectMacrophage-
Palavras-chave: dc.subjectPeritonitis-
Palavras-chave: dc.subjectSynthetic peptides-
Título: dc.titleEffects of Formyl Peptide Receptor Agonists Ac9-12 and WKYMV in In Vivo and In Vitro Acute Inflammatory Experimental Models-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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