Modulation of Epithelial Mesenchymal Transition after AGTR-1 Gene Edition by Crispr/Cas9 and Losartan Treatment in Mammary Tumor Cell Line: A Comparative Study between Human and Canine Species

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Autor(es): dc.contributor(FAMERP)-
Autor(es): dc.contributorUniversidade Paulista (UNIP)-
Autor(es): dc.contributorFaculdade de Medicina de São José do Rio Preto (FAMERP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorMoschetta-Pinheiro, Marina Gobbe-
Autor(es): dc.creatorColombo, Jucimara-
Autor(es): dc.creatorde Godoy, Bianca Lara Venâncio-
Autor(es): dc.creatorBalan, Julia Ferreira-
Autor(es): dc.creatorNascimento, Bianca Carlos-
Autor(es): dc.creatorZuccari, Debora Aparecida Pires de Campos-
Data de aceite: dc.date.accessioned2025-08-21T17:06:57Z-
Data de disponibilização: dc.date.available2025-08-21T17:06:57Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2021-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/life11121427-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/223187-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/223187-
Descrição: dc.descriptionBreast cancer is the most prevalent tumor type among women and female dogs. Tumor malignancy is characterized by the epithelial-to-mesenchymal transition (EMT) which leads to the metastasis formation. The inhibition of angiotensin II type I receptor (AGTR1) by an antagonist such as losartan can suppress angiogenesis, consequently contributing to the metastasis control. The aim of this study was to analyze the capacity of losartan and AGTR-1 gene edition to modulate the EMT process in triple negative/metastatic mammary tumor cells, compared to existing treatment protocols such as carboplatin. The cell lines CF41.Mg and MDA-MB-468, were cultured and treated with carboplatin, losartan, or submitted to AGTR-1 gene edition by CRISPR/Cas9. EMT markers and PARP-1 protein and gene expression were evaluated by immunofluorescence or immunocytochemistry and qRT-PCR, respectively. Cell migration capacity was also evaluated. For CF41.Mg and MDA-MB-468 cell lines, there was an increase in E-cadherin and a decrease in N-cadherin and PARP-1 protein and gene expression after treatment with carboplatin, losartan, both in combination and after AGTR-1 gene edition. There was a decrease in VEGF and PARP-1 protein and gene expression after AGTR-1 gene edition. Moreover, in both lines, reduction in invasion rate was observed after all treatments. Our data suggest that losartan and the gene edition of AGTR-1 by CRISPR/Cas9 were able to block the DNA repair and control the EMT process, such as carboplatin. The results in the canine species are unprecedented, as there are no data in the literature that demonstrate the action of losartan in this tumor type.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionPostGraduate Program in Health Sciences Faculdade de Medicina de São José do Rio Preto (FAMERP), Avenida Brigadeiro Faria Lima, 5416-
Descrição: dc.descriptionDepartment of Health Sciences Universidade Paulista (UNIP), Avenida Juscelino K. de Oliveira, s/n-
Descrição: dc.descriptionLaboratório de Investigação Molecular no Câncer (LIMC) Faculdade de Medicina de São José do Rio Preto (FAMERP), Avenida Brigadeiro Faria Lima, 5416-
Descrição: dc.descriptionPostGraduate Program in Genetics Instituto de Biociências Letras e Ciências Exatas (UNESP/IBILCE), Rua Cristovão Colombo, 2265-
Descrição: dc.descriptionPostGraduate Program in Genetics Instituto de Biociências Letras e Ciências Exatas (UNESP/IBILCE), Rua Cristovão Colombo, 2265-
Descrição: dc.descriptionFAPESP: 2017/15006-5-
Idioma: dc.languageen-
Relação: dc.relationLife-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAGTR-1-
Palavras-chave: dc.subjectGene edition-
Palavras-chave: dc.subjectLosartan-
Palavras-chave: dc.subjectMammary tumors-
Palavras-chave: dc.subjectTriple negative cell lines-
Título: dc.titleModulation of Epithelial Mesenchymal Transition after AGTR-1 Gene Edition by Crispr/Cas9 and Losartan Treatment in Mammary Tumor Cell Line: A Comparative Study between Human and Canine Species-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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