Correlating biological activity to thermo-structural analysis of the interaction of CTX with synthetic models of macrophage membranes

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorInstituto Butantan-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorde Araújo Pimenta, Luciana-
Autor(es): dc.creatorDuarte, Evandro L.-
Autor(es): dc.creatorMuniz, Gabriel S. Vignoli-
Autor(es): dc.creatorPasqualoto, Kerly Fernanda Mesquita-
Autor(es): dc.creatorde Mattos Fontes, Marcos Roberto-
Autor(es): dc.creatorLamy, M. Teresa-
Autor(es): dc.creatorSampaio, Sandra Coccuzzo-
Data de aceite: dc.date.accessioned2025-08-21T21:58:43Z-
Data de disponibilização: dc.date.available2025-08-21T21:58:43Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2021-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-021-02552-0-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/223019-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/223019-
Descrição: dc.descriptionThe important pharmacological actions of Crotoxin (CTX) on macrophages, the main toxin in the venom of Crotalus durissus terrificus, and its important participation in the control of different pathophysiological processes, have been demonstrated. The biological activities performed by macrophages are related to signaling mediated by receptors expressed on the membrane surface of these cells or opening and closing of ion channels, generation of membrane curvature and pore formation. In the present work, the interaction of the CTX complex with the cell membrane of macrophages is studied, both using biological cells and synthetic lipid membranes to monitor structural alterations induced by the protein. Here we show that CTX can penetrate THP-1 cells and induce pores only in anionic lipid model membranes, suggesting that a possible access pathway for CTX to the cell is via lipids with anionic polar heads. Considering that the selectivity of the lipid composition varies in different tissues and organs of the human body, the thermostructural studies presented here are extremely important to open new investigations on the biological activities of CTX in different biological systems.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionPathophysiology Laboratory Instituto Butantan, Av. Vital Brazil, 1500-
Descrição: dc.descriptionDepartment of Pharmacology Institute of Biomedical Sciences University of São Paulo, Av. Lineu Prestes, 1524-
Descrição: dc.descriptionInstitute of Physics University of São Paulo, R. Do Matão, 1371-
Descrição: dc.descriptionAlchemy Inovação Pesquisa e Desenvolvimento Ltda. Technology-Based Business Incubator of São Paulo IPEN/USP – Cietec, Av. Lineu Prestes, 2242 – Building D-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences Paulista State University, Prof. Dr Antonio Celso Wagner Zanin, 250-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences Paulista State University, Prof. Dr Antonio Celso Wagner Zanin, 250-
Descrição: dc.descriptionCNPq: 158259/2018-1-
Descrição: dc.descriptionFAPESP: 2014/50983-3-
Descrição: dc.descriptionFAPESP: 2017/11161-6-
Descrição: dc.descriptionCNPq: 302883/2017-7-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Título: dc.titleCorrelating biological activity to thermo-structural analysis of the interaction of CTX with synthetic models of macrophage membranes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.