Use Low Ozone Dosages has Positive Effects on the Cooling and Cryopreservation of Equine Semen

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de Mato Grosso do Sul (UFMS)-
Autor(es): dc.creatorPereira, Raiza R-
Autor(es): dc.creatorNogueira, Bruno G-
Autor(es): dc.creatorMilan, Bruno-
Autor(es): dc.creatorAcacio, Bianca R-
Autor(es): dc.creatorFreitas- Dell'Aqua, Camila P-
Autor(es): dc.creatorSouza, Maria IL-
Autor(es): dc.creatorSampaio, Breno FB-
Data de aceite: dc.date.accessioned2025-08-21T21:08:41Z-
Data de disponibilização: dc.date.available2025-08-21T21:08:41Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2021-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jevs.2021.103800-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/222925-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/222925-
Descrição: dc.descriptionThe aim of this study was to determine an ozone dosage capable of inducing pro-oxidation, and to verify its action on sperm cells during the process of cooling and cryopreservation of equine semen. In this study, we evaluated the ozone concentrations of 2µg/mL,15µg/mL, 30µg/mL e 60 µg/mL added in equine semen cooling and freezing extenders. Samples were evaluated for sperm kinetics patterns, function of sperm structures and lipid peroxidation. In the experiment, the concentration of 15 µg/mL showed higher total and progressive motility when comparing to control (60.3±3 and 40.7±3.4 vs. 54.9±4 e 35.0±4.4, respectively, P < .05) at M24 of cooling; The concentration of 2 µg/mL showed higher percentage of intact plasma and acrosomal membrane when comparing to control at M24 (51.1±3.6 vs. 46.1±3.9, P < .05), M24 after 30 minutes of incubation (43.4±3.1 versus 32.4±2.6, P <.05). The concentration of 2 µg/mL showed higher percentage of intact plasma and acrosomal membrane (P <.05) comparing to control at moments M0 (43.5±5.0 vs. 36.3±3.5), M30 (41.0±3,7 vs. 35.3±2,9) e M60 (39.0±7.0 vs. 31.4±5.4). Thus, it can be concluded that low doses of ozone can lead to a positive response in the sperm kinetics patterns and sperm structures after sperm storage at low temperatures. Higher concentrations (30 and 60 µg/mL) were harmful in the cooling and cryopreservation of equine semen.-
Descrição: dc.descriptionSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP)-
Descrição: dc.descriptionVeterinary Medicine and Animal Science of the Federal University of Mato Grosso do Sul Foundation - FAMEZ/UFMS-
Descrição: dc.descriptionSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationJournal of Equine Veterinary Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectLipid peroxidation-
Palavras-chave: dc.subjectSpermatozoa-
Palavras-chave: dc.subjectstallion-
Título: dc.titleUse Low Ozone Dosages has Positive Effects on the Cooling and Cryopreservation of Equine Semen-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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