Immobilization of the Tannase From Aspergillus fumigatus CAS21: Screening the Best Derivative for the Treatment of Tannery Effluent Using a Packed Bed Reactor

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorCavalcanti, Rayza Morganna Farias-
Autor(es): dc.creatorMaestrello, Chadia Chahud-
Autor(es): dc.creatorGuimarães, Luis Henrique Souza-
Data de aceite: dc.date.accessioned2025-08-21T17:13:12Z-
Data de disponibilização: dc.date.available2025-08-21T17:13:12Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2021-11-02-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fbioe.2021.754061-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/222895-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/222895-
Descrição: dc.descriptionEnzyme immobilization is an important alternative to stabilize enzyme properties favoring the efficiency of derivatives (enzyme + support/matrix) for different purposes. According to this, the current study aimed to immobilize the Aspergillus fumigatus CAS21 tannase and the use of the derivatives in the treatment of the effluent produced by the tannery industry. The tannase was immobilized on sodium alginate, DEAE-Sephadex, amberlite, and glass pearls as supports. Calcium alginate was the most adequate support for tannase immobilization with 100% yield and 94.3% for both efficiency and activity. The best tannase activity for the calcium alginate derivative was obtained at 50°C–60°C and pH 5.0. Thermal and pH stabilities evaluated for 24 h at 30°C–60°C and pH 4–7, respectively, were improved if compared to the stability of the free enzyme. Considering the reuse of the calcium alginate derivative, 78% of the initial activity was preserved after 10 catalytic cycles, and after the 9-month storage at 4°C, the activity was maintained in 70%. This derivative was applied in a packed bed reactor (PBR) for the treatment of tannin-rich effluents from the tannery industry. The reduction of the tannin content was effective reaching degradation of 74–78% after 48 h of PBR operation. The concentration of total phenolic compounds was also reduced, and the color and clarity of the effluent improved. In conclusion, the calcium alginate derivative is an attractive alternative as biocatalyst for large-scale treatment of the effluents from the tannery industry.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionInstituto de Química de Araraquara—UNESP-
Descrição: dc.descriptionDepartamento de Biologia Faculdade de Filosofia Ciências e Letras de Ribeirão Preto—USP-
Descrição: dc.descriptionInstituto de Química de Araraquara—UNESP-
Descrição: dc.descriptionFAPESP: 2016/11311-5-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Bioengineering and Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectalginate beads-
Palavras-chave: dc.subjecteffluent treatment-
Palavras-chave: dc.subjectenzymatic reactor-
Palavras-chave: dc.subjecttannery wastewater-
Palavras-chave: dc.subjecttannin acyl hydrolase-
Título: dc.titleImmobilization of the Tannase From Aspergillus fumigatus CAS21: Screening the Best Derivative for the Treatment of Tannery Effluent Using a Packed Bed Reactor-
Tipo de arquivo: dc.typelivro digital-
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