Impacts of high-intensity exercise on the metabolomics profile of human skeletal muscle tissue

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorVictoria University-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.creatorZagatto, Alessandro M.-
Autor(es): dc.creatorBishop, David J.-
Autor(es): dc.creatorAntunes, Barbara Moura-
Autor(es): dc.creatorBeck, Wladimir R.-
Autor(es): dc.creatorMalta, Elvis S.-
Autor(es): dc.creatorde Poli, Rodrigo A.B.-
Autor(es): dc.creatorCavaglieri, Claudia R.-
Autor(es): dc.creatorChacon-Mikahil, Mara Patrícia T.-
Autor(es): dc.creatorCastro, Alex-
Data de aceite: dc.date.accessioned2025-08-21T19:47:15Z-
Data de disponibilização: dc.date.available2025-08-21T19:47:15Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/sms.14086-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/222796-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/222796-
Descrição: dc.descriptionThe study aimed to identify and quantify the metabolites profile and metabolic pathways in human muscle tissue engaged during exhaustive high-intensity cycling exercise. Seven healthy physically active men performed a graded exercise test and an exhaustive supramaximal effort at 115% of maximal aerobic power with muscles biopsies performed in rest and immediately after exhaustion for quantifying of muscle metabolites changes by 1H-NMR spectroscopy. The time until exhaustion (tlim) recorded was 224.7 ± 35.5 s whereas the muscle pH at exhaustion was 6.48 ± 0.05. A total of 54 metabolites were identified and quantified. The most enriched and impacted pathways included: beta oxidation of very long chain fatty acids, mitochondrial electron transport chain, alanine aspartate, and glutamate metabolism, citric acid cycle, arginine biosynthesis, propanoate metabolism, threonine and 2-oxobutanoate degradation and pyruvate metabolism. In addition, the muscle concentrations in Post exercise, compared to Pre increased significantly (p < 0.0398) for fumarate (42.0%), succinate (101.2%), glucose (249.7%), lactate (122.8%), O-acetylcarnitine (164.7%), glycerol (79.3%), AMP (288.2%), 2-oxobutyrate (121.0%), and methanol (58.5%), whereas decreased significantly (p < 0.010) for creatine phosphate (−70.2%), ADP (−56.5%), carnitine (−33.5%), and glutamate (−42.3%). Only the succinate was significantly correlated with tlim (r = −0.76; p = 0.0497). Besides the classical expected contribution of glycolytic and phosphagen energetic pathways, it was demonstrated that the high-intensity exercise is also associated with pathways indicatives of amino acid and fatty acid oxidation metabolisms, highlighting the inverse relation between changes in the intramuscular succinate levels and tlim.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionLaboratory of Physiology and Sport Performance (LAFIDE) São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute for Health and Sport (iHeS) Victoria University-
Descrição: dc.descriptionFederal University of Sao Carlos (UFSCar)-
Descrição: dc.descriptionLaboratory of Exercise Physiology Faculty of Physical Education University of Campinas (UNICAMP)-
Descrição: dc.descriptionLaboratory of Physiology and Sport Performance (LAFIDE) São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2017/17445-1-
Descrição: dc.descriptionFAPESP: 2017/21724-8-
Descrição: dc.descriptionFAPESP: 2018/21126-6-
Descrição: dc.descriptionFAPESP: 2019/25282-5-
Descrição: dc.descriptionFAPESP: 2020/13939-7-
Descrição: dc.descriptionCNPq: 307719/2016-2-
Formato: dc.format402-413-
Idioma: dc.languageen-
Relação: dc.relationScandinavian Journal of Medicine and Science in Sports-
???dc.source???: dc.sourceScopus-
Título: dc.titleImpacts of high-intensity exercise on the metabolomics profile of human skeletal muscle tissue-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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