The CRISPR toolbox for the gram-positive model bacterium Bacillus subtilis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorZocca, Vitoria Fernanda Bertolazzi-
Autor(es): dc.creatorCorrêa, Graciely Gomes-
Autor(es): dc.creatorLins, Milca Rachel da Costa Ribeiro-
Autor(es): dc.creatorde Jesus, Victor Nunes-
Autor(es): dc.creatorTavares, Leonardo Ferro-
Autor(es): dc.creatorAmorim, Laura Araujo da Silva-
Autor(es): dc.creatorKundlatsch, Guilherme Engelberto-
Autor(es): dc.creatorPedrolli, Danielle Biscaro-
Data de aceite: dc.date.accessioned2025-08-21T21:57:29Z-
Data de disponibilização: dc.date.available2025-08-21T21:57:29Z-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2022-04-28-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/07388551.2021.1983516-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/222794-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/222794-
Descrição: dc.descriptionCRISPR has revolutionized the way we engineer genomes. Its simplicity and modularity have enabled the development of a great number of tools to edit genomes and to control gene expression. This powerful technology was first adapted to Bacillus subtilis in 2016 and has been intensely upgraded since then. Many tools have been successfully developed to build a CRISPR toolbox for this Gram-positive model and important industrial chassis. The toolbox includes tools, such as double-strand and single-strand cutting CRISPR for point mutation, gene insertion, and gene deletion up to 38 kb. Moreover, catalytic dead Cas proteins have been used for base editing, as well as for the control of gene expression (CRISPRi and CRISPRa). Many of these tools have been used for multiplex CRISPR with the most successful one targeting up to six loci simultaneously for point mutation. However, tools for efficient multiplex CRISPR for other functionalities are still missing in the toolbox. CRISPR engineering has already resulted in efficient protein and metabolite-producing strains, demonstrating its great potential. In this review, we cover all the important additions made to the B. subtilis CRISPR toolbox since 2016, and strain developments fomented by the technology.-
Descrição: dc.descriptionDepartment of Bioprocess Engineering and Biotechnology School of Pharmaceutical Sciences Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionDepartment of Bioprocess Engineering and Biotechnology School of Pharmaceutical Sciences Universidade Estadual Paulista (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationCritical Reviews in Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBacillus subtilis-
Palavras-chave: dc.subjectbacteria-
Palavras-chave: dc.subjectCRISPR-
Palavras-chave: dc.subjectgene editing-
Palavras-chave: dc.subjectgene expression activation-
Palavras-chave: dc.subjectgene expression interference-
Palavras-chave: dc.subjectgenome editing-
Palavras-chave: dc.subjectregulation of gene expression-
Palavras-chave: dc.subjectsynthetic biology-
Palavras-chave: dc.subjecttoolbox-
Título: dc.titleThe CRISPR toolbox for the gram-positive model bacterium Bacillus subtilis-
Tipo de arquivo: dc.typevídeo-
Aparece nas coleções:Repositório Institucional - Unesp

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